Caspase-6 Activity Assay Kit

Name: Caspase-6 Activity Assay Kit
SKU: PTAK-007

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-007

Description

The Caspase-6 Assay Kit is a complete assay system designed to measure Caspase-6 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-6 activity measurements. In addition, the kit includes purified Caspase-6 enzyme and a potent Caspase-6 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-6 activity level. The fluorogenic substrate, Ac-VEID-AFC, is incubated with purified Caspase-6 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Applications

Great for studying enzyme kinetics and HTS applications.

Caution

Avoid freeze/thaw cycles

Component

•Caspase-6
•Caspase-6 assay buffer
•Caspase-6 substrate
•DTT
•Caspase-6 inhibitor (Ac-IETD-CHO)
•Microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is Caspase-6?

A: Caspase-6 is a type of enzyme that belongs to the caspase family. Caspases are a group of proteins involved in the process of apoptosis, which is programmed cell death.

What does caspase-6 do in apoptosis?

A: Caspase-6 is classified as an apoptotic effector protein that mediates nuclear shrinkage during apoptosis, but it has a unique activation and regulation mechanism that is different from other effector protein caspases.

What activates caspase-6?

A: Caspase-6 is normally activated by caspase-3 rather than the initiating caspase. However, it can also be activated in the absence of caspase-3 and has been reported to self-activate in vitro and in vivo.

What diseases are caspase-6 associated with?

A: Abnormal regulation or dysregulation of caspase-6 has been implicated in various diseases, including neurodegenerative disorders such as Alzheimer's disease and Huntington's disease, as well as certain types of cancer.

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