Cat.No. : CSC-RG1717 Host Cell: HEK293T
| Cat. No. | CSC-RG1717 |
| Background | This gene encodes a protein that exhibits G-protein coupled adenosine receptor activity. Adora2b receptor regulates renal medulla blood flow and sodium excretion and is involved in angiogenesis and blood vessel remodeling after injury. |
| Gene | ADORA2B |
| Host Cell | HEK293T |
| Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Source | HEK293T |
| Subgroup | Rat |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Gene Name | Adora2b adenosine A2B receptor [ Rattus norvegicus ] |
| Gene Symbol | Adora2b |
| Gene Description | adenosine A2B receptor |
| Gene ID | 29316 |
| UniProt ID | P29276 |
| mRNA Refseq | NM_017161.1 |
| Protein Refseq | NP_058857.1 |
| Chromosome Location | 10q23 |
| Function | G-protein coupled adenosine receptor activity; G-protein coupled receptor activity; |
| Pathway | Adenosine P1 receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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