Rat ADORA2B Stable Cell Line-HEK293T

Name: Rat ADORA2B Stable Cell Line-HEK293T
SKU: CSC-RG1717
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1717

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1717
Background	This gene encodes a protein that exhibits G-protein coupled adenosine receptor activity. Adora2b receptor regulates renal medulla blood flow and sodium excretion and is involved in angiogenesis and blood vessel remodeling after injury.
Target Gene	ADORA2B
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Source	HEK293T
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Adora2b adenosine A2B receptor [ Rattus norvegicus ]
Gene Symbol	Adora2b
Gene Description	adenosine A2B receptor
Gene ID	29316
Uni Prot ID	P29276
m RNA Refseq	NM_017161.1
Protein Refseq	NP_058857.1
Chromosome Location	10q23
Function	G-protein coupled adenosine receptor activity; G-protein coupled receptor activity;
Pathway	Adenosine P1 receptors, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem;

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