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Mouse Hrh3 Stable Cell Line-HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RG1734

Host Cell :   HEK293T Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No. CSC-RG1734
Background This genes encodes a protein that exhibits drug binding (ortholog) and histamine receptor activity (ortholog).
Target Gene Hrh3
Gene Species Mus musculus (Mouse)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Source HEK293T
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Research on the mechanisms of GPCR-related diseases

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name Hrh3 histamine receptor H3 [ Mus musculus (house mouse) ]
Gene Symbol Hrh3
Synonyms H3R; HH3R; AW049250
Gene ID 99296
Uni Prot ID P58406
Chromosome Location 2; 2 H4
Function G-protein coupled acetylcholine receptor activity; G-protein coupled receptor activity; drug binding; histamine receptor activity; signal transducer activity;
Pathway Amine ligand-binding receptors, organism-specific biosystem (from REACTOME); Class A/1 (Rhodopsin-like receptors), organism-specific biosystem (from REACTOME); G alpha (i) signalling events, organism-specific biosystem (from REACTOME); GPCR downstream signaling, organism-specific biosystem (from REACTOME); GPCR ligand binding, organism-specific biosystem (from REACTOME); Histamine receptors, organism-specific biosystem (from REACTOME); Neuroactive ligand-receptor interaction, organism-specific biosystem (from KEGG);
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