Human XCR1 Stable Cell Line-CHO-K1

Human XCR1 encodes a seven-transmembrane chemokine receptor enriched in cross-presenting dendritic-cell subsets. Its activation by XCL1 and XCL2 initiates Gi/o-linked pathways associated with chemotaxis and immune-cell positioning, making receptor abundance and cellular context important determinants of experimental response. This product is a stable CHO-K1 cell line engineered to maintain elevated expression of human XCR1. This cell line is engineered to stably overexpress Human XCR1 in CHO-K1 cells. CHO-K1 cells offer robust growth, a comparatively low endogenous background for many human receptors, and a long history of use in pharmacology and screening. Stable integration reduces the experiment-to-experiment variability associated with transient transfection and supports repeated use across assay campaigns. The product therefore provides a defined cellular reagent in which receptor-dependent readouts can be compared with suitable parental, mock-transfected, or pathway controls.

Functionally, the line is intended to provide consistent access to human XCR1-dependent biology. Depending on the selected detection system, receptor activity may be evaluated through ligand binding, calcium mobilization, cAMP modulation, membrane-proximal signaling, receptor internalization, chemotaxis, transcriptional reporters, or other validated downstream endpoints. It is particularly suitable for ligand binding, receptor activation, migration-related signaling, antibody characterization, and discovery of agents that modulate dendritic-cell recruitment or antigen-presentation biology. Because stable expression can differ from native expression in receptor density, coupling efficiency, and regulatory feedback, users should optimize ligand concentration, incubation time, cell density, and assay controls for the intended endpoint.