Human VTCN1 Stable Cell Line-CHO-K1

Name: Human VTCN1 Stable Cell Line-CHO-K1
SKU: CSC-RO0003
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0003

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0003
Description	This cell line is engineered to stably overexpress the human VTCN1 in CHO-K1 cells.
Background	This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
Target Gene	VTCN1
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	VTCN1 V-set domain containing T cell activation inhibitor 1 [ Homo sapiens ]
Gene Symbol	VTCN1
Synonyms	VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member; H4; B7 superfamily member 1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7 family member, H4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4;
Gene ID	79679
Uni Prot ID	Q7Z7D3
m RNA Refseq	BC065717
Chromosome Location	1p12
Function	receptor binding;
MIM	608162

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Q & A

Customer Reviews

Customer Q&As

Using Human VTCN1 Stable Cell Line-CHO, how can the regulatory role of VTCN1 protein on T cell immune responses be evaluated?

A: By co-culturing Human VTCN1 Stable Cell Line-CHO with T cells and monitoring T cell activation and proliferation, the regulatory role of VTCN1 protein on T cell immune responses can be evaluated.

Does the expression level of VTCN1 protein in Human VTCN1 Stable Cell Line-CHO vary with cell cycle stages?

A: The expression level of VTCN1 protein in Human VTCN1 Stable Cell Line-CHO may be influenced by cell cycle stages, which requires validation through experiments such as flow cytometry.

In Human VTCN1 Stable Cell Line-CHO, how can the glycosylation modification of VTCN1 protein be quantitatively analyzed?

A: Utilizing specific glycosylation antibodies or mass spectrometry methods can quantitatively analyze the glycosylation modification of VTCN1 protein in Human VTCN1 Stable Cell Line-CHO.

Is Human VTCN1 Stable Cell Line-CHO suitable for studying the signaling mechanisms of VTCN1 protein?

A: Yes, Human VTCN1 Stable Cell Line-CHO is highly suitable for studying the signaling mechanisms of VTCN1 protein, including its interactions with downstream effector molecules.

How to assess the biosafety and genetic stability of Human VTCN1 Stable Cell Line-CHO?

A: Through molecular and cellular biology experiments such as PCR, Southern blot, and cytotoxicity tests, the biosafety and genetic stability of Human VTCN1 Stable Cell Line-CHO can be assessed.

Is Human VTCN1 Stable Cell Line-CHO suitable for conducting anti-cancer drug screening research?

A: Yes, given the association of VTCN1 protein with immune suppression, Human VTCN1 Stable Cell Line-CHO can be utilized for anti-cancer drug screening research targeting the VTCN1 pathway.

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