Cat.No. : CSC-RO0003 Host Cell: CHO-K1
| Cat. No. | CSC-RO0003 |
| Description | This cell line is engineered to stably overexpress the human VTCN1 in CHO-K1 cells. |
| Background | This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene | VTCN1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Gene Name | VTCN1 V-set domain containing T cell activation inhibitor 1 [ Homo sapiens ] |
| Gene Symbol | VTCN1 |
| Synonyms | VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member; H4; B7 superfamily member 1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7 family member, H4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4; |
| Gene ID | 79679 |
| UniProt ID | Q7Z7D3 |
| mRNA Refseq | BC065717 |
| Chromosome Location | 1p12 |
| Function | receptor binding; |
| MIM | 608162 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: By co-culturing Human VTCN1 Stable Cell Line-CHO with T cells and monitoring T cell activation and proliferation, the regulatory role of VTCN1 protein on T cell immune responses can be evaluated.
A: The expression level of VTCN1 protein in Human VTCN1 Stable Cell Line-CHO may be influenced by cell cycle stages, which requires validation through experiments such as flow cytometry.
A: Utilizing specific glycosylation antibodies or mass spectrometry methods can quantitatively analyze the glycosylation modification of VTCN1 protein in Human VTCN1 Stable Cell Line-CHO.
A: Yes, Human VTCN1 Stable Cell Line-CHO is highly suitable for studying the signaling mechanisms of VTCN1 protein, including its interactions with downstream effector molecules.
A: Through molecular and cellular biology experiments such as PCR, Southern blot, and cytotoxicity tests, the biosafety and genetic stability of Human VTCN1 Stable Cell Line-CHO can be assessed.
A: Yes, given the association of VTCN1 protein with immune suppression, Human VTCN1 Stable Cell Line-CHO can be utilized for anti-cancer drug screening research targeting the VTCN1 pathway.
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