Cat.No. : CSC-RO0073 Host Cell: CHO-K1
| Cat. No. | CSC-RO0073 |
| Description | This cell line is a stably transfected cell line which expresses mouse V-set domain containing T cell activation inhibitor 1 (Vtcn1). |
| Background | Mice homozygous for this mutation display stronger Th1 responses upon parasitic infection by L. major including reduced footpad swelling and lower parasite burden compared to controls. Responses to other Th1-driven immune responses are normal. |
| Gene | Vtcn1 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Target | Mouse |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Vtcn1 gene encodes for a protein that is involved in the regulation of the cytoskeleton and vesicle trafficking. In the Mouse Vtcn1 Stable Cell Line-CHO (Chinese Hamster Ovary), the stable expression of Vtcn1 allows for the study of its role in cell morphology, cell division, and the transport of proteins and lipids within the cell.
A: By providing a controlled environment where Vtcn1 is stably expressed, researchers can model the effects of Vtcn1 mutations on cellular processes. This cell line can be used to investigate the pathogenesis of diseases linked to Vtcn1, such as neurological disorders, and to test potential therapeutic interventions.
A: The CHO (Chinese Hamster Ovary) cells are known for their ability to produce large quantities of recombinant proteins. The Mouse Vtcn1 Stable Cell Line-CHO can be used for high-throughput screening to identify compounds that modulate Vtcn1 activity, which could lead to the development of new drugs targeting Vtcn1-related diseases.
A: With the stable expression of Vtcn1, researchers can perform co-immunoprecipitation and other biochemical assays to identify and characterize proteins that interact with Vtcn1. This can provide insights into the cellular pathways in which Vtcn1 is involved.
A: Although CHO cells are not mouse cells, they can be engineered to express mouse genes like Vtcn1. This allows researchers to study the function of mouse proteins in a cell type that is amenable to genetic manipulation and has a well-characterized physiology.
A: The understanding of Vtcn1's role in cell morphology and cytoskeleton dynamics could inform strategies for tissue engineering and cell-based therapies. The Mouse Vtcn1 Stable Cell Line-CHO could be used to test the effects of Vtcn1 modulation on cell behavior relevant to regenerative medicine.
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