Human TNFSF18 Stable Cell Line-HEK293T

Name: Human TNFSF18 Stable Cell Line-HEK293T
SKU: CSC-RO0101
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0101

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0101
Description	HEK293T-TNFSF18 cell line is a stably transfected cell line which expresses human TNF superfamily member 18 (TNFSF18).
Target Gene	TNFSF18
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFSF18 tumor necrosis factor (ligand) superfamily, member 18 [ Homo sapiens ]
Gene Symbol	TNFSF18
Synonyms	TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TL6; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand; GITRL; MGC138237;
GeneID	8995
Uni ProtID	Q9UNG2
mRNA Refseq	BC069319
Chromosome Location	1q23
Function	cytokine activity; receptor binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
MIM	603898

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Customer Q&As

What is the origin of Human TNFSF18 Stable Cell Line-HEK293T, and how was it established?

A: Human TNFSF18 Stable Cell Line-HEK293T is derived from the HEK293T (Human Embryonic Kidney 293T) cell line and has been genetically modified to stably express the human TNFSF18 (Tumor Necrosis Factor Superfamily Member 18) gene. Typically, this cell line is established by transfecting the TNFSF18 gene construct into HEK293T cells, followed by selection and maintenance of stable expression.

What is the genotype and phenotype of Human TNFSF18 Stable Cell Line-HEK293T?

A: The genotype of Human TNFSF18 Stable Cell Line-HEK293T includes the genetic makeup of HEK293T cells, along with stable expression of the human TNFSF18 gene. In terms of phenotype, these cells express the TNFSF18 protein, which is a cell surface receptor known to be involved in immune regulation and signaling.

What are the primary applications of Human TNFSF18 Stable Cell Line-HEK293T in scientific research and drug development?

A: Human TNFSF18 Stable Cell Line-HEK293T has several applications, including studying the interaction of TNFSF18 with its ligands, investigating immune checkpoint pathways, evaluating potential immunotherapies, and screening molecules that modulate TNFSF18 signaling. It is valuable for research related to immune regulation and the development of immunotherapies.

Is the use of Human TNFSF18 Stable Cell Line-HEK293T subject to specific regulatory and ethical restrictions?

A: Yes, the use of Human TNFSF18 Stable Cell Line-HEK293T is subject to regulatory and ethical guidelines, especially when working with human-derived cell lines in research. Researchers are expected to adhere to relevant ethical standards, obtain necessary approvals, and ensure compliance with applicable regulations to ensure ethical compliance of experiments.

What potential challenges may arise when using Human TNFSF18 Stable Cell Line-HEK293T in research?

A: Challenges associated with using Human TNFSF18 Stable Cell Line-HEK293T in research may include maintaining stable expression of TNFSF18, conducting experiments that accurately represent complex immune checkpoint pathways, and ensuring the relevance of research findings to clinical applications. Careful experimental design and data interpretation are crucial.

How can Human TNFSF18 Stable Cell Line-HEK293T contribute to the development of immunotherapies or cancer treatments?

A: Human TNFSF18 Stable Cell Line-HEK293T can contribute to the development of immunotherapies and cancer treatments by allowing researchers to study the TNFSF18 signaling pathway and potential immunomodulators. This research can provide valuable insights into how to intervene in the immune system to combat cancer.

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