Cat.No. : CSC-RO0101 Host Cell: HEK293T
| Cat. No. | CSC-RO0101 |
| Description | HEK293T-TNFSF18 cell line is a stably transfected cell line which expresses human TNF superfamily member 18 (TNFSF18). |
| Gene | TNFSF18 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human TNFSF18 Stable Cell Line-HEK293T is derived from the HEK293T (Human Embryonic Kidney 293T) cell line and has been genetically modified to stably express the human TNFSF18 (Tumor Necrosis Factor Superfamily Member 18) gene. Typically, this cell line is established by transfecting the TNFSF18 gene construct into HEK293T cells, followed by selection and maintenance of stable expression.
A: The genotype of Human TNFSF18 Stable Cell Line-HEK293T includes the genetic makeup of HEK293T cells, along with stable expression of the human TNFSF18 gene. In terms of phenotype, these cells express the TNFSF18 protein, which is a cell surface receptor known to be involved in immune regulation and signaling.
A: Human TNFSF18 Stable Cell Line-HEK293T has several applications, including studying the interaction of TNFSF18 with its ligands, investigating immune checkpoint pathways, evaluating potential immunotherapies, and screening molecules that modulate TNFSF18 signaling. It is valuable for research related to immune regulation and the development of immunotherapies.
A: Yes, the use of Human TNFSF18 Stable Cell Line-HEK293T is subject to regulatory and ethical guidelines, especially when working with human-derived cell lines in research. Researchers are expected to adhere to relevant ethical standards, obtain necessary approvals, and ensure compliance with applicable regulations to ensure ethical compliance of experiments.
A: Challenges associated with using Human TNFSF18 Stable Cell Line-HEK293T in research may include maintaining stable expression of TNFSF18, conducting experiments that accurately represent complex immune checkpoint pathways, and ensuring the relevance of research findings to clinical applications. Careful experimental design and data interpretation are crucial.
A: Human TNFSF18 Stable Cell Line-HEK293T can contribute to the development of immunotherapies and cancer treatments by allowing researchers to study the TNFSF18 signaling pathway and potential immunomodulators. This research can provide valuable insights into how to intervene in the immune system to combat cancer.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
