Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RG0358
Host Cell : CHO dhfr- Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RG0358 |
| Background | Prolactin-releasing peptide receptor PRRP (or GPR10) is detected in human brain, pituitaries, normal portions of adrenal glands and various tumor tissues. High level of expression is detected in pheochromocytomas. GPR10 was investigated regarding the effect of normal and high-fat diets on energy intake, body weight, and glucose homeostasis in wild-type and GPR10 knockout mice. The investigation provided direct evidence that GPR10 is involved in the regulation of energy balance. |
| Target Gene | PRLHR |
| Gene Species | Homo sapiens (Human) |
| Abbr | CHO dhfr--HuPRLHR |
| Alias | PRLHR, GPR10, hGR3, GR3, PrRPR, MGC126539, MGC126541 |
| Host Cell | CHO dhfr- |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | PRLHR prolactin releasing hormone receptor [ Homo sapiens ] |
| Gene Symbol | PRLHR |
| Synonyms | GR3; GPR10; PrRPR |
| Gene Description | prolactin releasing hormone receptor |
| Gene ID | 2834 |
| Uni Prot ID | A5JUU5 |
| m RNA Refseq | NM_004248.2 |
| Protein Refseq | NP_004239.1 |
| Chromosome Location | 10q26.13 |
| Function | G-protein coupled receptor activity; neuropeptide Y receptor activity; neuropeptide receptor activity; peptide hormone binding; |
| Pathway | Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem; Peptide ligand-binding receptors, organism-specific biosystem; Signal Transduction, organism-specific biosystem; |
| MIM | 600895 |
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