Human KCNJ11/ABCC9 Stable Cell Line-HEK293

Name: Human KCNJ11/ABCC9 Stable Cell Line-HEK293
SKU: CSC-RI0120
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0120

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0120
Description	This cell line is engineered to overexpress human ABCC9.
Background	The human KCNJ11 gene encodes the pore-forming subunit of heteromeric Kir6.2/SUR2A, an ATP-sensitive, inwardly rectifyiing potassium channel. ABCC9 encodes the SUR2A subunit which confers sulfonylurea sensitivity. Kir6.2/SUR2A channels expressed in heart are therapeutic targets for cardiac ischemia.
Target Gene	ABCC9
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNJ11/ABCC9
Alias	ABCC9, ABC37, ATFB12, CANTU, CMD1O, SUR2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ABCC9 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 [ Homo sapiens ]
Gene Symbol	ABCC9
Synonyms	SUR2; ABC37; CANTU; CMD1O; ATFB12
Gene Description	ATP-binding cassette, sub-family C (CFTR/MRP), member 9
GeneID	10060
Uni ProtID	O60706
mRNA Refseq	NM_005691.2
Protein Refseq	NP_005682.2
Chromosome Location	12p12.1
Function	ATP binding; ATPase activity, coupled to transmembrane movement of substances; ion channel binding; potassium channel regulator activity; sulfonylurea receptor activity; transporter activity;
Pathway	ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; ABC-family proteins mediated transport, organism-specific biosystem; ATP sensitive Potassium channels, organism-specific biosystem; Inwardly rectifying K+ channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	601439

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