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Human GPR142 Stable Cell Line-HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RG1742

Host Cell :   HEK293T Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RG1742
Background GPR142 is a member of the rhodopsin family of G protein-coupled receptors (GPRs).
Target Gene GPR142
Gene Species Homo sapiens (Human)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Source HEK293T
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Research on the mechanisms of GPCR-related diseases

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name GPR142 G protein-coupled receptor 142 [ Homo sapiens ]
Gene Symbol GPR142
Synonyms PGR2
Gene Description G protein-coupled receptor 142
Gene ID 350383
Uni Prot ID Q7Z601
m RNA Refseq NM_181790.1
Protein Refseq NP_861455.1
Chromosome Location 17q25.1
Function G-protein coupled receptor activity;
MIM 609046
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