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Cat.No. : CSC-RG0032 Host Cell: U2OS
| Cat. No. | CSC-RG0032 |
| Background | GLP2 receptor (GLP2R) is a G protein-coupled receptor superfamily member related to glucagon receptor and GLP1 receptor. Glucagon like peptide 2 receptor is expressed in SNC and gut. GLP2R transduces the biological actions of GLP-2, a 33 aminoacid peptide produced by the intestinal endocrine L cell and by various neurons in the central nervous system. Intestinal GLP-2 is co-secreted along with GLP-1 upon nutrient ingestion. GLP-2 produces several actions such as including intestinal growth, enhancement of intestinal function, reduction in bone breakdown and neuroprotection. |
| Gene | GLP2R |
| Gene Species | Homo sapiens (Human) |
| Alias | GLP2R, GLP-2R, GLP-2-R |
| Host Cell | U2OS |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human GLP2R-tGFP Stable Cell Line-U2OS is a valuable tool for studying the function and regulation of the glucagon-like peptide-2 receptor (GLP2R), a GPCR involved in various physiological processes. By stably expressing the GLP2R fused with a green fluorescent protein (tGFP), researchers can visualize the receptor's subcellular localization and activity in response to ligand stimulation, facilitating studies on receptor trafficking, signaling pathways, and drug interactions.
A: This cell line enables high-throughput screening of potential drugs that modulate GLP2R activity. By monitoring changes in tGFP fluorescence, researchers can assess the efficacy of compounds in activating or inhibiting the receptor, aiding in the discovery of new therapeutics for conditions such as diabetes and obesity.
A: Ensuring the long-term expression and functionality of the GLP2R-tGFP fusion protein can be challenging due to factors like gene silencing, chromosomal integration site effects, and proteasomal degradation. Regular monitoring of the cell line's phenotype and functional assays are crucial to maintain its stability.
A: By providing a system where GLP2R activity can be directly observed, this cell line allows researchers to investigate the downstream signaling events triggered by receptor activation. This includes studying the involvement of second messenger systems, protein-protein interactions, and gene expression changes.
A: When using this cell line for safety testing, it's important to consider the potential off-target effects of drugs on the tGFP fluorescence, which could interfere with the readout. Additionally, the cell line's response to drugs should be compared with other cell lines and in vivo models to ensure the relevance of findings.
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