Human GLP2R-tGFP Stable Cell Line-U2OS

Name: Human GLP2R-tGFP Stable Cell Line-U2OS
SKU: CSC-RG0032
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0032

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0032
Background	GLP2 receptor (GLP2R) is a G protein-coupled receptor superfamily member related to glucagon receptor and GLP1 receptor. Glucagon like peptide 2 receptor is expressed in SNC and gut. GLP2R transduces the biological actions of GLP-2, a 33 aminoacid peptide produced by the intestinal endocrine L cell and by various neurons in the central nervous system. Intestinal GLP-2 is co-secreted along with GLP-1 upon nutrient ingestion. GLP-2 produces several actions such as including intestinal growth, enhancement of intestinal function, reduction in bone breakdown and neuroprotection.
Target Gene	GLP2R
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuGLP2R-tGFP
Alias	GLP2R, GLP-2R, GLP-2-R
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GLP2R glucagon-like peptide 2 receptor [ Homo sapiens ]
Gene Symbol	GLP2R
Gene Description	glucagon-like peptide 2 receptor
GeneID	9340
Uni ProtID	O95838
mRNA Refseq	NM_004246.1
Protein Refseq	NP_004237.1
Chromosome Location	17p13.3
Function	G-protein coupled receptor activity; glucagon receptor activity;
Pathway	Class B/2 (Secretin family receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class B Secretin-like, organism-specific biosystem; Glucagon-type ligand receptors, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem;
MIM	603659

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What is the specific utility of the Human GLP2R-tGFP Stable Cell Line-U2OS in studying G protein-coupled receptors (GPCRs)?

A: The Human GLP2R-tGFP Stable Cell Line-U2OS is a valuable tool for studying the function and regulation of the glucagon-like peptide-2 receptor (GLP2R), a GPCR involved in various physiological processes. By stably expressing the GLP2R fused with a green fluorescent protein (tGFP), researchers can visualize the receptor's subcellular localization and activity in response to ligand stimulation, facilitating studies on receptor trafficking, signaling pathways, and drug interactions.

How does the Human GLP2R-tGFP Stable Cell Line-U2OS contribute to the development of therapeutics targeting GLP2R?

A: This cell line enables high-throughput screening of potential drugs that modulate GLP2R activity. By monitoring changes in tGFP fluorescence, researchers can assess the efficacy of compounds in activating or inhibiting the receptor, aiding in the discovery of new therapeutics for conditions such as diabetes and obesity.

What are the challenges in maintaining the stability of the GLP2R-tGFP fusion protein in the Human GLP2R-tGFP Stable Cell Line-U2OS?

A: Ensuring the long-term expression and functionality of the GLP2R-tGFP fusion protein can be challenging due to factors like gene silencing, chromosomal integration site effects, and proteasomal degradation. Regular monitoring of the cell line's phenotype and functional assays are crucial to maintain its stability.

How does the Human GLP2R-tGFP Stable Cell Line-U2OS aid in understanding the role of GLP2R in cell signaling?

A: By providing a system where GLP2R activity can be directly observed, this cell line allows researchers to investigate the downstream signaling events triggered by receptor activation. This includes studying the involvement of second messenger systems, protein-protein interactions, and gene expression changes.

What are the considerations for using the Human GLP2R-tGFP Stable Cell Line-U2OS in drug safety testing?

A: When using this cell line for safety testing, it's important to consider the potential off-target effects of drugs on the tGFP fluorescence, which could interfere with the readout. Additionally, the cell line's response to drugs should be compared with other cell lines and in vivo models to ensure the relevance of findings.

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