Cat.No. : CSC-RG0044 Host Cell: HEK293
| Cat. No. | CSC-RG0044 |
| Background | This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. |
| Gene | CXCR7 |
| Gene Species | Homo sapiens (Human) |
| Alias | CXCR7, CMKOR1, GPR159, RDC1, RDC-1, CMKOR1, CXC-R7, CXCR-7 |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CXCR7-SNAP Stable Cell Line-HEK293 achieves the stability of the SNAP tag by genetically engineering the fusion of the SNAP tag (a protein tag) with the gene of CXCR7 protein, followed by the selection of cells that stably express this fusion protein through a selective medium. Stability is verified through long-term culture and monitoring the expression levels of the SNAP tag.
A: During drug screening, key parameters to consider include the expression level of the CXCR7 receptor, the functionality of the SNAP tag, the cell proliferation rate, and the cell's response to drug treatment.
A: This cell line can be used to study the activation of the CXCR7 receptor, the identification of downstream signaling molecules, and key regulatory events in the signaling pathways. Additionally, it can be used to screen small molecules or biologics that affect CXCR7 signaling.
A: The advantages of this cell line lie in its stable expression of the SNAP tag, which allows it to be used in HTS to quickly identify compounds that interact with the CXCR7 receptor, thereby accelerating the drug discovery process.
A: Reliability can be assessed through multiple experiments, including verifying the functionality of the SNAP tag with CXCR7, monitoring the stability of the cell phenotype, and the cell line's performance in drug screening experiments. Additionally, the background variation and batch-to-batch consistency of the cell line should be considered.
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