Human CXCR7-SNAP Stable Cell Line-HEK293

Name: Human CXCR7-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0044
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0044

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-RG0044
Background	This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.
Target Gene	CXCR7
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCXCR7-SNAP
Alias	CXCR7, CMKOR1, GPR159, RDC1, RDC-1, CMKOR1, CXC-R7, CXCR-7
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CXCR7 chemokine (C-X-C motif) receptor 7 [ Homo sapiens ]
Gene Symbol	CXCR7
Synonyms	CXCR7; chemokine (C-X-C motif) receptor 7; chemokine orphan receptor 1 , CMKOR1; C-X-C chemokine receptor type 7; GPR159; RDC1; G protein-coupled receptor; chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog; RDC-1; CMKOR1; CXC-R7; CXCR-7;
Gene ID	57007
Uni Prot ID	P25106
m RNA Refseq	BC036661
Chromosome Location	2q37.3
Function	C-X-C chemokine receptor activity; G-protein coupled receptor activity; receptor activity; signal transducer activity;
Pathway	Chemokine receptors bind chemokines, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem;
MIM	610376

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How does the Human CXCR7-SNAP Stable Cell Line-HEK293 ensure the stability of the SNAP tag?

A: The Human CXCR7-SNAP Stable Cell Line-HEK293 achieves the stability of the SNAP tag by genetically engineering the fusion of the SNAP tag (a protein tag) with the gene of CXCR7 protein, followed by the selection of cells that stably express this fusion protein through a selective medium. Stability is verified through long-term culture and monitoring the expression levels of the SNAP tag.

What are the key parameters to consider when using the Human CXCR7-SNAP Stable Cell Line-HEK293 for drug screening?

A: During drug screening, key parameters to consider include the expression level of the CXCR7 receptor, the functionality of the SNAP tag, the cell proliferation rate, and the cell's response to drug treatment.

What are the potential applications of the Human CXCR7-SNAP Stable Cell Line-HEK293 in studying the signaling pathways of the CXCR7 receptor?

A: This cell line can be used to study the activation of the CXCR7 receptor, the identification of downstream signaling molecules, and key regulatory events in the signaling pathways. Additionally, it can be used to screen small molecules or biologics that affect CXCR7 signaling.

What are the advantages of the Human CXCR7-SNAP Stable Cell Line-HEK293 in high-throughput screening (HTS)?

A: The advantages of this cell line lie in its stable expression of the SNAP tag, which allows it to be used in HTS to quickly identify compounds that interact with the CXCR7 receptor, thereby accelerating the drug discovery process.

How can the reliability of the Human CXCR7-SNAP Stable Cell Line-HEK293 in drug development be assessed?

A: Reliability can be assessed through multiple experiments, including verifying the functionality of the SNAP tag with CXCR7, monitoring the stability of the cell phenotype, and the cell line's performance in drug screening experiments. Additionally, the background variation and batch-to-batch consistency of the cell line should be considered.

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