Human CHRM4/Rat Kcnj3/Mouse Kcnj6 Stable Cell Line-HEK293

Name: Human CHRM4/Rat Kcnj3/Mouse Kcnj6 Stable Cell Line-HEK293
SKU: CSC-RG0879
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0879

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0879
Background	Stimulation of Gi-coupled receptors in heart and brain activates G-protein-gated inward rectifier K+ channels (GIRK) via Gbetagamma (Dascal, N. 2001). This activity can be detected using a GIRK-mediated thallium flux assay described by Niswender et al., 2008. This assay is compatible with HTS and represents a novel and a sensitive assay for detecting Gi/o -coupled GPCRs.
Target Gene	Kcnj6
Abbr	HEK293-HuCHRM4/rKcnj3/mKcnj6
Alias	KCNJ6, GIRK-2, wv, BIR1, GIRK2, KATP2, KCNJ7, Kir3.2, weaver
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Kcnj6 potassium inwardly-rectifying channel, subfamily J, member 6 [ Mus musculus ]
Gene Symbol	Kcnj6
Synonyms	wv; BIR1; GIRK2; KATP2; KCNJ7; Kir3.2; weaver
Gene Description	potassium inwardly-rectifying channel, subfamily J, member 6
GeneID	16522
Uni ProtID	P48542
mRNA Refseq	NM_001025590.1
Protein Refseq	NP_001020761.1
Chromosome Location	16 C4; 16 68.75 cM
Function	G-protein activated inward rectifier potassium channel activity; G-protein alpha-subunit binding; inward rectifier potassium channel activity; ion channel activity; voltage-gated ion channel activity;
Pathway	Activation of G protein gated Potassium channels, organism-specific biosystem; Activation of GABAB receptors, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; G protein gated Potassium channels, organism-specific biosystem; GABA B receptor activation, organism-specific biosystem;

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