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Human Druggable Genome siRNA Library enables efficient drug target screening.
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Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
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Powerful Tn5 Transposase for DNA insertion and random library construction.
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CGT Biosafety Testing
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Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
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AI-Antibody Engineering Fusion
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AI-Driven Enzyme Engineering
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High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
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Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
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Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Cat.No. : CSC-RG0879 Host Cell: HEK293
| Cat. No. | CSC-RG0879 |
| Background | Stimulation of Gi-coupled receptors in heart and brain activates G-protein-gated inward rectifier K+ channels (GIRK) via Gbetagamma (Dascal, N. 2001). This activity can be detected using a GIRK-mediated thallium flux assay described by Niswender et al., 2008. This assay is compatible with HTS and represents a novel and a sensitive assay for detecting Gi/o -coupled GPCRs. |
| Gene | Kcnj6 |
| Alias | KCNJ6, GIRK-2, wv, BIR1, GIRK2, KATP2, KCNJ7, Kir3.2, weaver |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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