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Cat.No. : CSC-RO0078 Host Cell: HEK293T
| Cat. No. | CSC-RO0078 |
| Description | HEK293T-BTLA cell line is a stably transfected cell line which expresses human B and T lymphocyte associated (BTLA). |
| Gene | BTLA |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human BTLA Stable Cell Line-HEK293T is derived from the HEK293T (Human Embryonic Kidney 293T) cell line and has been engineered to stably express the human BTLA (B and T lymphocyte attenuator) gene. This cell line is typically established through transfection of HEK293T cells with a construct containing the BTLA gene, followed by selection and maintenance of stable expression.
A: Yes, the use of Human BTLA Stable Cell Line-HEK293T is subject to regulatory and ethical guidelines that govern the use of human-derived cell lines in research. Researchers should adhere to relevant ethical standards, obtain necessary approvals, and ensure compliance with applicable regulations when working with this cell line.
A: Human BTLA Stable Cell Line-HEK293T should be stored in liquid nitrogen for long-term preservation. To revive these cells, they can be quickly thawed at 37 degrees Celsius and seeded into culture dishes using appropriate growth medium and conditions.
A: When working with Human BTLA Stable Cell Line-HEK293T, researchers should follow general biosafety guidelines for handling human-derived cell lines. This includes working in appropriate biosafety cabinets, using personal protective equipment, and following established laboratory safety protocols to prevent contamination and ensure safe handling.
A: In scientific research with Human BTLA Stable Cell Line-HEK293T, researchers should consider the following: Ensure proper experimental design and controls to obtain reliable results.
Regularly confirm the stability of BTLA expression and cell phenotype.
Use appropriate culture conditions and media to maintain cell health.
A: Human BTLA Stable Cell Line-HEK293T can be a valuable tool in researching immunotherapies and cancer treatments by allowing researchers to study the BTLA signaling pathway and potential modulators of immune checkpoint receptors. This knowledge can aid in the development of targeted therapies and immunomodulatory agents.
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