Human BTLA Stable Cell Line-HEK293T

Name: Human BTLA Stable Cell Line-HEK293T
SKU: CSC-RO0078
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0078

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0078
Description	HEK293T-BTLA cell line is a stably transfected cell line which expresses human B and T lymphocyte associated (BTLA).
Target Gene	BTLA
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BTLA B and T lymphocyte associated [ Homo sapiens ]
Gene Symbol	BTLA
Synonyms	BTLA1; CD272
Gene Description	B and T lymphocyte associated
Gene ID	151888
Uni Prot ID	Q7Z6A9
m RNA Refseq	NM_181780.3
Protein Refseq	NP_861445.3
Chromosome Location	3q13.2
Function	receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem;
MIM	607925

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the origin of Human BTLA Stable Cell Line-HEK293T, and how was it established?

A: Human BTLA Stable Cell Line-HEK293T is derived from the HEK293T (Human Embryonic Kidney 293T) cell line and has been engineered to stably express the human BTLA (B and T lymphocyte attenuator) gene. This cell line is typically established through transfection of HEK293T cells with a construct containing the BTLA gene, followed by selection and maintenance of stable expression.

Are there specific regulatory and ethical considerations associated with the use of Human BTLA Stable Cell Line-HEK293T?

A: Yes, the use of Human BTLA Stable Cell Line-HEK293T is subject to regulatory and ethical guidelines that govern the use of human-derived cell lines in research. Researchers should adhere to relevant ethical standards, obtain necessary approvals, and ensure compliance with applicable regulations when working with this cell line.

What are the recommended methods for the storage and revival of Human BTLA Stable Cell Line-HEK293T?

A: Human BTLA Stable Cell Line-HEK293T should be stored in liquid nitrogen for long-term preservation. To revive these cells, they can be quickly thawed at 37 degrees Celsius and seeded into culture dishes using appropriate growth medium and conditions.

Are there any specific biosafety precautions to be taken when handling Human BTLA Stable Cell Line-HEK293T?

A: When working with Human BTLA Stable Cell Line-HEK293T, researchers should follow general biosafety guidelines for handling human-derived cell lines. This includes working in appropriate biosafety cabinets, using personal protective equipment, and following established laboratory safety protocols to prevent contamination and ensure safe handling.

What are the key considerations and precautions for scientific research involving Human BTLA Stable Cell Line-HEK293T?

A: In scientific research with Human BTLA Stable Cell Line-HEK293T, researchers should consider the following: Ensure proper experimental design and controls to obtain reliable results.<br/>Regularly confirm the stability of BTLA expression and cell phenotype.<br/>Use appropriate culture conditions and media to maintain cell health.

How can Human BTLA Stable Cell Line-HEK293T contribute to the development of immunotherapies or cancer treatments?

A: Human BTLA Stable Cell Line-HEK293T can be a valuable tool in researching immunotherapies and cancer treatments by allowing researchers to study the BTLA signaling pathway and potential modulators of immune checkpoint receptors. This knowledge can aid in the development of targeted therapies and immunomodulatory agents.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *