Human BTLA Stable Cell Line-CHO-S

Name: Human BTLA Stable Cell Line-CHO-S
SKU: CSC-RO1028
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1028

Host Cell : CHO-S Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1028
Description	CHO-S-BTLA cell line is engineered to stably overexpress human BTLA.
Target Gene	BTLA
Gene Species	Homo sapiens (Human)
Host Cell	CHO-S
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BTLA B and T lymphocyte associated [ Homo sapiens ]
Gene Symbol	BTLA
Synonyms	BTLA1; CD272
Gene Description	B and T lymphocyte associated
Gene ID	151888
Uni Prot ID	Q7Z6A9
m RNA Refseq	NM_181780.3
Protein Refseq	NP_861445.3
Chromosome Location	3q13.2
Function	receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem;
MIM	607925

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How was the Human BTLA Stable Cell Line-CHO-S established?

A: The cell line was established by stably transfecting the human BTLA (B and T lymphocyte attenuator) gene into CHO-S cells.

What is the application of the Human BTLA Stable Cell Line-CHO-S in immune checkpoint therapy research?

A: This cell line can be used to study the role of BTLA in immune checkpoint therapies, especially in regulating immune responses and cancer treatment.

What should be considered when using the Human BTLA Stable Cell Line-CHO-S for drug screening?

A: It is important to consider the drug's impact on BTLA expression and function, while ensuring that the screening conditions do not lead to nonspecific cellular responses.

What is the importance of the Human BTLA Stable Cell Line-CHO-S in studying immune tolerance?

A: Since BTLA is closely related to immune tolerance, this cell line is significant for understanding and modulating immune tolerance mechanisms.

How to ensure the stability and functionality of the Human BTLA Stable Cell Line-CHO-S during use?

A: The cells should be cultured in a specific medium containing selective antibiotics and regularly checked for BTLA protein expression and functionality.

What is the application of the Human BTLA Stable Cell Line-CHO-S in cell signaling research?

A: It can be used to study the cell signaling pathways mediated by BTLA, especially in the activation and inhibition of immune cells.

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