Human ADRB3 Stable Cell Line-HEK293

Name: Human ADRB3 Stable Cell Line-HEK293
SKU: CSC-RG0004
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0004

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0004
Background	The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRbeta3 mediates in lipolysis in the adipose tissue and in thermogenesis in the skeletal muscle. Some beta3 agonists have shown antidepressant effects in animal studies.
Target Gene	ADRB3
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuADRB3
Alias	ADRB3, BETA3AR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ADRB3 adrenergic, beta-3-, receptor [ Homo sapiens ]
Gene Symbol	ADRB3
Synonyms	ADRB3; adrenergic, beta-3-, receptor; beta-3 adrenergic receptor; beta-3 adrenoceptor; beta-3 adrenoreceptor; BETA3AR; FLJ99960;
Gene ID	155
Uni Prot ID	P13945
Chromosome Location	8p12
Function	G-protein coupled receptor activity; beta-3 adrenergic receptor binding; beta-adrenergic receptor activity; beta3-adrenergic receptor activity; epinephrine binding; contributes_to norepinephrine binding; protein binding; protein homodimerization activity; receptor activity; signal transducer activity;
Pathway	Adrenoceptors, organism-specific biosystem; Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Endocytosis, organism-specific biosystem;
MIM	109691

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Customer Reviews

Customer Q&As

How is the Human ADRB3 Stable Cell Line-HEK293 constructed through genetic engineering?

A: The Human ADRB3 Stable Cell Line-HEK293 is created by genetically engineering HEK293 cells to stably express the human β3 adrenergic receptor (ADRB3) gene. This process typically involves cloning the ADRB3 gene into an expression vector and then transfecting this vector into HEK293 cells. Through selection and cloning, cells that stably express ADRB3 are chosen, forming a stable cell line.

How is the stability of the Human ADRB3 Stable Cell Line-HEK293 assessed in research?

A: Stability is assessed by long-term monitoring of the expression levels of the ADRB3 gene in the cell line, as well as observing the cell's phenotypic and functional characteristics. This may involve techniques such as real-time quantitative PCR, Western Blot, and immunofluorescence staining to quantitatively and qualitatively analyze the expression of ADRB3. Additionally, the cell's morphology, growth characteristics, and drug responses are regularly checked to ensure stability.

What are the applications of the Human ADRB3 Stable Cell Line-HEK293 in drug screening?

A: The cell line can be used for screening and evaluating drugs targeting the ADRB3 receptor, such as β3 adrenergic receptor agonists or antagonists. By treating these cells with drugs, researchers can observe the effects of the drugs on the signaling pathways mediated by ADRB3, thus assessing the efficacy and selectivity of potential therapies.

What is the role of the Human ADRB3 Stable Cell Line-HEK293 in high-throughput screening (HTS)?

A: The cell line can serve as a screening platform for rapidly identifying small molecules that affect the function of ADRB3. Through automated experimental processes, a large number of compounds can be tested in a short time, accelerating the drug discovery process.

How is the Human ADRB3 Stable Cell Line-HEK293 used in drug toxicity studies?

A: The cell line can be used to assess the potential toxicity of drugs on ADRB3-expressing cells. By monitoring cell viability, apoptosis, and cell cycle changes after drug treatment, researchers can evaluate the safety and side effects of drugs.

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