Cat.No. : CSC-RG0004 Host Cell: HEK293
| Cat. No. | CSC-RG0004 |
| Background | The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. ADRbeta3 mediates in lipolysis in the adipose tissue and in thermogenesis in the skeletal muscle. Some beta3 agonists have shown antidepressant effects in animal studies. |
| Gene | ADRB3 |
| Gene Species | Homo sapiens (Human) |
| Alias | ADRB3, BETA3AR |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human ADRB3 Stable Cell Line-HEK293 is created by genetically engineering HEK293 cells to stably express the human β3 adrenergic receptor (ADRB3) gene. This process typically involves cloning the ADRB3 gene into an expression vector and then transfecting this vector into HEK293 cells. Through selection and cloning, cells that stably express ADRB3 are chosen, forming a stable cell line.
A: Stability is assessed by long-term monitoring of the expression levels of the ADRB3 gene in the cell line, as well as observing the cell's phenotypic and functional characteristics. This may involve techniques such as real-time quantitative PCR, Western Blot, and immunofluorescence staining to quantitatively and qualitatively analyze the expression of ADRB3. Additionally, the cell's morphology, growth characteristics, and drug responses are regularly checked to ensure stability.
A: The cell line can be used for screening and evaluating drugs targeting the ADRB3 receptor, such as β3 adrenergic receptor agonists or antagonists. By treating these cells with drugs, researchers can observe the effects of the drugs on the signaling pathways mediated by ADRB3, thus assessing the efficacy and selectivity of potential therapies.
A: The cell line can serve as a screening platform for rapidly identifying small molecules that affect the function of ADRB3. Through automated experimental processes, a large number of compounds can be tested in a short time, accelerating the drug discovery process.
A: The cell line can be used to assess the potential toxicity of drugs on ADRB3-expressing cells. By monitoring cell viability, apoptosis, and cell cycle changes after drug treatment, researchers can evaluate the safety and side effects of drugs.
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