Human ADRA1D Stable Cell Line-CHO-K1

Name: Human ADRA1D Stable Cell Line-CHO-K1
SKU: CSC-RG1646
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1646

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1646
Background	Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1D-adrenergic receptor. Similar to alpha-1B-adrenergic receptor gene, this gene comprises 2 exons and a single intron that interrupts the coding region.
Target Gene	ADRA1D
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Source	CHO-K1
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ADRA1D adrenoceptor alpha 1D [ Homo sapiens ]
Gene Symbol	ADRA1D
Synonyms	DAR; ADRA1; ADRA1A; ADRA1R; ALPHA1; dJ779E11.2
Gene Description	adrenergic, alpha-1D-, receptor
Gene ID	146
Uni Prot ID	B0ZBE0
m RNA Refseq	NM_000678.3
Protein Refseq	NP_000669.1
Chromosome Location	20p13
Function	alpha1-adrenergic receptor activity;
Pathway	Adrenoceptors, organism-specific biosystem; Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (12/13) signalling events, organism-specific biosystem;
MIM	104219

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