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rAAV-EF1a-fDIO-mCherry-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-8

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Virus Particles Information

Cat. No. RABVH-8
Description Flp-dependent AAV helper co-expressing mCherry and TVA via T2A under EF1a. mCherry marks Flp-expressing starter cells in red and TVA restricts entry of EnvA-pseudotyped G-deleted rabies virus to those cells. When supplemented with RVG, the virus crosses one synapse retrogradely, allowing red-labelled, Flp-specific monosynaptic input networks to be mapped.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Flp-dependent
Helper Function AAV helper co-expressing mCherry and TVA under Flp-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing with red starter labeling
  2. mCherry and TVA co-expressed via T2A self-cleaving peptide
  3. fDIO restricts expression to Flp+ populations
  4. Dual-color experiments with EGFP-labeled rabies virus
  5. Mapping direct presynaptic inputs to Flp-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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