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rAAV-EF1a-DIO-TVA(E66T)-P2A-EGFP

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-15

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Virus Particles Information

Cat. No. RABVH-15
Description Cre-dependent AAV helper expressing TVA(E66T) and EGFP via a P2A peptide under EF1a. The low-affinity receptor restricts entry of EnvA-pseudotyped G-deleted rabies virus to Cre-expressing starter neurons while limiting background infection. EGFP provides green starter-cell labelling, and co-supplied RVG enables single-synapse retrograde mapping of direct inputs.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Cre-dependent
Helper Function AAV helper expressing low-affinity TVA(E66T) and EGFP under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with green starter labeling
  2. TVA(E66T) reduces background starter cell artifacts
  3. P2A enables bicistronic expression
  4. DIO restricts expression to Cre+ populations
  5. Improved specificity in starter cell identification
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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