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rAAV-EF1a-DIO-H2B-EGFP-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-20

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Virus Particles Information

Cat. No. RABVH-20
Description Cre-dependent AAV helper co-expressing nuclear-localized H2B-EGFP and TVA through T2A under EF1a. Nuclear EGFP gives sharply defined labelling of Cre-positive starter neurons, and TVA restricts entry of EnvA-pseudotyped G-deleted rabies virus to those cells. Used with an RVG helper, it enables Cre-dependent retrograde monosynaptic mapping of direct presynaptic inputs.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Cre-dependent
Helper Function AAV helper expressing nuclear-localized EGFP and TVA under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with nuclear green starter labeling
  2. H2B fusion confines EGFP to nucleus for unambiguous starter cell identification
  3. T2A enables bicistronic expression
  4. DIO restricts expression to Cre+ populations
  5. Mapping direct presynaptic inputs to Cre-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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