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rAAV-CaMKIIa-EGFP-F2A-TVA-T2A-N2cG

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-61

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Virus Particles Information

Cat. No. RABVH-61
Description Excitatory-neuron-specific all-in-one AAV helper co-expressing EGFP, TVA and the CVS-N2c glycoprotein (N2cG) via F2A/T2A under CaMKIIa. TVA permits entry of EnvA-pseudotyped G-deleted rabies virus and N2cG complements it with high efficiency and low toxicity, allowing one-step retrograde mapping of inputs onto EGFP-labelled excitatory neurons from a single injection.
Product Type Adeno-associated virus
Reporter EGFP
Helper Function AAV helper co-expressing EGFP, TVA, and N2cG in excitatory neurons
Applications
  1. Co-injection with CVS-N2c-EnvA-ΔG RV for constitutive monosynaptic retrograde tracing in excitatory neurons
  2. CaMKIIα restricts to excitatory pyramidal neurons
  3. N2cG for higher transsynaptic efficiency and lower toxicity
  4. EGFP for green starter visualization
  5. Mapping direct presynaptic inputs to excitatory projection neurons with reduced toxicity
Promoter Neuron-specific Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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