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Human CLEC5A Stable Cell Line - CHOK1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO02450

Host Cell :   CHO-K1 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO02450
Description This cell line is engineered to stably overexpress Human CLEC5A in CHOK1 cells.
Product Type Stable cell line constitutively expressing target gene(s)
Target Gene CLEC5A
Gene Species Human
Host Cell CHO-K1
Host Cell Species Cricetulus griseus (Chinese hamster)
Applications 1) Biological function research of the target gene(s),
2) Drug development of potential drug candidates targeting the target gene(s) or protein(s),
3) Investigation of disease mechanisms,
4) Antibody validation
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry,
2) Mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene CLEC5A
Background This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein interacts with dnax-activation protein 12 and may play a role in cell activation. Alternative splice variants have been described but their full-length sequence has not been determined. [provided by RefSeq, Jul 2008]
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