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Cat. No. : RSNK-027
| Cat. No. | RSNK-027 |
| Description | Poly(A) Polymerase catalyzes the transfer of AMP to 3′-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+ as cofactor. |
| Source | An E. coli strain that carries the Poly (A) Polymerase gene. |
| Concentration | 5 U/μl |
| Applications |
• Adding a poly(A) tail to RNA • 3′-end RNA labeling |
| Size | 750 U, 5 KU |
| Storage | Store at -20 °C |
A: Poly-A polymerase is an enzyme that adds a poly-A tail to a messenger RNA molecule during the process of RNA processing.
A: On mRNA, the poly(A) tail protects the mRNA molecule from enzymatic degradation in the cytoplasm and facilitates transcription termination, mRNA export from the nucleus, and translation.
A: The molecular weight of Poly(A) polymerase can vary depending on the specific type and organism. Generally, it ranges from about 55 to 86 kilodaltons.
A: Poly(A) polymerase can be inactivated by adding EDTA to a final concentration of 10 mM in the reaction buffer.
A: No. Poly(A) polymerase will not add A's to single-stranded DNA.
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The Poly (A) Polymerase can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequences. It is very helpful for our research.
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