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Poly (A) Polymerase

For research use only. Not intended for any clinical use.
Cat.No.
RSNK-027
Description
Poly(A) Polymerase catalyzes the transfer of AMP to 3′-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+ as cofactor.
Applications
• Adding a poly(A) tail to RNA
• 3′-end RNA labeling
Concentration
5 U/μl
Size/Form
750 U, 5 KU
Source
An E. coli strain that carries the Poly (A) Polymerase gene.
Storage
Store at -20 °C

Publications

Q & A

Customer Reviews

Customer Q&As
What is the Poly (A) Polymerase?

A: Poly-A polymerase is an enzyme that adds a poly-A tail to a messenger RNA molecule during the process of RNA processing.

What is the function of the poly A in mRNA?

A: On mRNA, the poly(A) tail protects the mRNA molecule from enzymatic degradation in the cytoplasm and facilitates transcription termination, mRNA export from the nucleus, and translation.

What is the molecular weight of Poly(A) polymerase?

A: The molecular weight of Poly(A) polymerase can vary depending on the specific type and organism. Generally, it ranges from about 55 to 86 kilodaltons.

How can the Poly(A) Polymerase be inactivated without heating up the reaction?

A: Poly(A) polymerase can be inactivated by adding EDTA to a final concentration of 10 mM in the reaction buffer.

Can Poly(A) Polymerase be used to add a poly A tail to ssDNA?

A: No. Poly(A) polymerase will not add A's to single-stranded DNA.

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Customer Reviews
It is very helpful for our research.

The Poly (A) Polymerase can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequences. It is very helpful for our research.

United States

01/27/2022

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