Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : RSNK-018
| Cat. No. | RSNK-018 |
| Description | Pseudo-UTP could be used to develop drugable mRNAs. Replacing the standard bases by modified ones can enhance their translation properties while reducing the immunogenicity. |
| Alias | Ψ-UTP, Pseudouridine-5'-Triphosphate |
| Applications | In vitro transcription |
| Molecular Formula | C9H15N2O15P3 |
| Molecular Weight | 484.1 |
| Size | 1 µmol, 5 µmol |
| Purity | ≥95% |
| Storage | Store at -20°C. |
A: Pseudouridine-5'-triphosphate (ΨTP or Pseudo-UTP) is a modified nucleoside triphosphate that contains a "pseudo" or "false" uridine base. It is used as a substrate for RNA polymerases to incorporate Ψ into RNA during transcription.
A: Ψ is the most common modified nucleoside found in RNA, and it is formed by converting uridine (U) via an isomerization reaction.
A: Ψ has been shown to play an important role in RNA structure and function, as it can enhance stability and regulate gene expression.
A: Uridine contains a nitrogenous base called uracil, while pseudouridine contains a modified form of uridine. In pseudouridine, the carbon-carbon bond between the ribose sugar and the nitrogenous base is modified, which results in a slightly different chemical structure.
A: In RNA, pseudouridine can help stabilize the structure of molecules and participate in various functions, including splicing, translation, and RNA degradation.
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Incorporation of ΨTP into RNA transcripts has been shown to improve translation efficiency of the RNA.
Great buy! ΨTP incorporation into RNA transcripts can enhance the accuracy of translation by reducing frameshift errors.
I like it. Unmodified RNA can stimulate the immune system and trigger an inflammatory response, whereas ΨTP-modified RNA is less likely to elicit an immune response.
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