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T7 High Yield RNA Synthesis Kit

For research use only. Not intended for any clinical use.
Cat.No.
RSNK-001
Description
T7 High Yield RNA Synthesis Kit is designed to perform in vitro RNA transcription experiment using T7 RNA polymerase. RNA synthesized using this kit can be used in a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, et al.
Applications
• In vitro translation
• Aantisense RNA and RNAi experiments
• RNA vaccines
• RNA structure and function studies
Size/Form
25 rxns
Storage
Store at -20°C.

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Q & A

Customer Reviews

Customer Q&As
What is T7 RNA polymerase?

A: T7 RNA polymerase is a type of enzyme that catalyzes the transcription of DNA into RNA. It is derived from the T7 bacteriophage, which is a virus that infects bacteria.

What is T7 RNA polymerase used for?

A: T7 RNA polymerase is widely used in molecular biology research, particularly in the field of gene expression studies. It is commonly used to produce large amounts of RNA from DNA templates for use in experiments such as in vitro translation, RNA interference, and gene therapy.

Why is T7 RNA polymerase so popular in research?

A: One of the reasons why T7 RNA polymerase is so popular in research is that it has high specificity for the T7 promoter sequence, which means that it selectively transcribes DNA sequences that begins with this specific sequence. This makes it easy to produce large amounts of RNA from specific genes of interest.

What is the source of T7 RNA polymerase?

A: T7 RNAP is a small (99 kDa) DNA-dependent RNAP derived from bacteriophage T7. The polymerase efficiently and specifically transcribes genes bearing a T7 promoter (PT7).

How can I improve on a low yield of RNA from the transcription reaction?

A: If the transcription reaction with the template produces full-length RNA, but the yield is significantly lower than expected, it may be that contaminants in the DNA template inhibit RNA polymerase, or the DNA concentration may be incorrect. Alternatively, additional purified DNA templates may be required.

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Customer Reviews
Useful Tool

The T7 High Yield RNA Synthesis Kit is designed for quick set-up and production of large amounts of RNA in vitro. The kit is a very useful tool.

United States

02/22/2023

Worked Very Well

Great kit. The reaction can be set up conveniently by combining the NTP buffer mix, T7 RNA Polymerase mix and a suitable DNA template.

United States

01/02/2021

Wide Range of Applications

Great buy! RNA synthesized with the kit can be used for RNA structure and function studies, as probes for RNase protection assays and hybridization based blots, anti-sense RNA and RNAi experiments, microinjection, as well as in vitro translation and RNA vaccines.

United States

03/25/2022

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