Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VPT4023
| Cat. No. | VPT4023 |
| Host Cell | TG1 |
| Tag | His6,SV5 |
| Promoter | M13,ColE |
| Resistance | Amp |
| Selection | Ampicillin |
| Vector Type | Phagemid vector |
| Vector Length | 4608bp |
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The pCDisplay-3 vector, with a length of 4608 base pairs (bp), is a type of phagemid vector that is used in genetic engineering and research for various applications related to gene cloning and protein expression. Hosted in TG1 cells, the pCDisplay-3 vector utilizes M13 and ColE promoters. In molecular biology, promoters are nucleotide sequences that act as a starting point for transcription where they facilitate the binding of RNA polymerase to DNA. The M13 and ColE promoters function to initiate the replication process for the vector, enhancing its capacity to express the desired gene or protein.
One of the critical features of the pCDisplay-3 vector is its antibiotic resistance, specifically to the drug Ampicillin. This resistance attribute is of crucial importance as it allows the identification and selection of successfully transformed cells in a bacterial population. When the vector, carrying an Ampicillin resistance gene, is introduced into bacteria, only those cells that have effectively incorporated the plasmid into their own genome can grow and survive in an Ampicillin-contained medium.
The pCDisplay-3 vector also comes equipped with His6 and SV5 tags. In genetic research, tags are short sequences of DNA that are attached to a gene or protein of interest to facilitate its identification or purification. The His6 tag enables the purification of the expressed protein of interest through immobilized metal affinity chromatography (IMAC), while the SV5 tag facilitates the detection and tracking of the protein produced within the cell.
The pCDisplay-3 vector, a phagemid vector, has various applications in molecular biology and genetic engineering. Its potential use is primarily derived from its ability to facilitate the display of foreign proteins or peptides on the surface of phage particles. This property is particularly valuable in fields such as vaccine development, protein engineering, and study of protein-protein interactions.
One of the main strengths of the pCDisplay-3 vector is its application in the engineering of novel proteins. Scientists can design and construct proteins to display on the surface of phage particles, which can then be screened for attractive characteristics. Such techniques can potentially be used to analyze proteins' capacity to bind with other proteins or to study their structural features. For example, small molecules and large macromolecules' interaction with proteins can be studied and selected for highest binding affinity through this method.
In addition, the pCDisplay-3 vector plays a significant role in vaccine development. It enables the presentation of viral or bacterial antigens on the phage surface, triggering specific immune responses. This strategy is being harnessed in the design of new vaccines, particularly for diseases difficult to treat with traditional vaccines.
Another critical application of the pCDisplay-3 vector is its potential use in drug design. It can be employed in high-throughput screening strategies to identify potential pharmaceutical compounds. These compounds' ability to bind to target proteins can be easily tested using this vector. It can be used to display a variety of peptides or protein fragments, allowing for a larger pool of potential ligands to be evaluated.
In conclusion, the pCDisplay-3 vector's suitable modifications due to its phage-display platform and advantages for vaccine development, protein engineering, drug design, and gene therapy make it an invaluable tool for researchers.
A: pCDisplay-3 is a phagemid vector can be used for the expression of scFv antibody.
A: For pCDisplay-3, the VL fragment can be cloned using the BssHII and SalI cut sites, while the VH fragment is cloned using the XhoI and NheI cut sites.
A: TG1 or Xl1-blue
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My experience with the pCDisplay-3 vector has been nothing short of outstanding. It's easy to handle and highly efficient. I highly recommend it to any researchers who require a robust phagemid vector.
I have been using pCDisplay-3 in my lab and it performs exceptionally well. It's highly reliable and contributes greatly to the accuracy of our research outcomes.
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