pCDisplay-2 vector

The pCDisplay-2 vector is a phagemid vector with a total length of 4612 base pairs, designed and developed chiefly for the purpose of phage display and antibody research. pCDisplay-2 displays several remarkable features and characteristics, making it an ideal tool for various biological research pursuits. The pCDisplay-2 phagemid vector utilizes the TG1 host strain. TG1 cells are known for their high transformation efficiency, making them a desirable host for plasmid amplification. This allows the pCDisplay-2 to reproduce substantially in the host strain, making it highly suitable for high-throughput studies. pCDisplay-2 vector contains M13 geneIII which is a component of the M13 bacteriophage. The protein produced by geneIII, often referred to as the pIII protein, is located on the surface of the phage and mediates the binding of the phage to the bacterial F pilus, which is important for the infection process. In addition to its role in infection, geneIII is also utilized in various molecular biology applications, including phage display, which allows for the study of protein interactions, antibody development, and the identification of peptides or proteins that can bind to specific targets. pCDisplay-2 vector is resistant to the antibiotic ampicillin (Amp), which makes it an interesting tool for the selection purposes. Moreover, pCDisplay-2 has two tags, His6 and Myc, which further increases its utility for a wide range of applications. Myc tags can be used for protein localization studies, co-immunoprecipitation, and chromatin immunoprecipitation. On the other hand, His6 tags are commonly employed for purifying proteins from the host cells, allowing for the easy extraction and detection of the protein of interest.