Cat.No. : CSC-RO0861 Host Cell: HEK293T
| Cat. No. | CSC-RO0861 |
| Description | This cell line is engineered to stably overexpress monkey CD93. |
| Gene | CD93 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey CD93 Stable Cell Line - HEK293T is derived from the HEK293T cell line, which is a human embryonic kidney cell line that has been extensively used in research. To generate this stable cell line expressing Monkey CD93, researchers likely transfected HEK293T cells with a plasmid containing the Monkey CD93 gene and a selection marker, such as a neomycin resistance gene. After transfection, cells expressing Monkey CD93 were selected and maintained under constant selection pressure, resulting in the establishment of the stable cell line.
A: The Monkey CD93 Stable Cell Line - HEK293T is likely used in scientific research to study the function and role of CD93 in monkeys, which is a cell surface receptor involved in processes like angiogenesis and immune regulation. Researchers may use this cell line for experiments related to cell adhesion, signal transduction, or immune response modulation. It can serve as a valuable tool for investigating Monkey CD93-specific functions and interactions.
A: The Monkey CD93 Stable Cell Line - HEK293T may have specific advantages compared to other cell lines expressing CD93. These advantages could include a stable and consistent expression of Monkey CD93, a well-characterized background (HEK293T), and the potential for use in various assays and experiments. Researchers should consider these characteristics when choosing a cell line for their specific research needs.
A: To maintain the Monkey CD93 Stable Cell Line - HEK293T, researchers should typically culture the cells in growth medium suitable for HEK293T cells, supplemented with the appropriate selection agent (e.g., neomycin). The specific culture conditions, such as temperature, CO2 concentration, and passage protocols, may depend on the established protocol used to generate and maintain this stable cell line. It's crucial to follow the instructions provided by the cell line provider or consult relevant literature for precise culture conditions.
A: When working with the Monkey CD93 Stable Cell Line - HEK293T, researchers should pay attention to several key factors: a. Regularly check and maintain the stability of Monkey CD93 expression. b. Ensure that the selection agent (e.g., neomycin) is present in the culture medium to prevent the loss of Monkey CD93-expressing cells. c. Perform proper quality control to confirm the identity and characteristics of the cell line. d. Avoid over-passage of the cells to prevent phenotypic changes. e. Document culture conditions and experimental procedures meticulously for reproducibility. f. Consider performing functional assays to validate Monkey CD93 functionality in the stable cell line.
A: Researchers should be aware of potential limitations when using the Monkey CD93 Stable Cell Line - HEK293T in research. These may include the possibility of cross-reactivity with antibodies designed for human CD93, as there could be differences in the antigenic regions between the monkey and human CD93 proteins. Additionally, researchers should consider that findings from experiments using this cell line may not always directly translate to humans due to species-specific differences. Careful validation and consideration of these factors are essential when interpreting results and drawing conclusions in a research context.
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