Monkey CD93 Stable Cell Line - HEK293T

Name: Monkey CD93 Stable Cell Line - HEK293T
SKU: CSC-RO0861
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0861

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0861
Description	This cell line is engineered to stably overexpress monkey CD93.
Target Gene	CD93
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the Monkey CD93 Stable Cell Line - HEK293T, and how was it established?

A: The Monkey CD93 Stable Cell Line - HEK293T is derived from the HEK293T cell line, which is a human embryonic kidney cell line that has been extensively used in research. To generate this stable cell line expressing Monkey CD93, researchers likely transfected HEK293T cells with a plasmid containing the Monkey CD93 gene and a selection marker, such as a neomycin resistance gene. After transfection, cells expressing Monkey CD93 were selected and maintained under constant selection pressure, resulting in the establishment of the stable cell line.

What are the specific applications of the Monkey CD93 Stable Cell Line - HEK293T in scientific research?

A: The Monkey CD93 Stable Cell Line - HEK293T is likely used in scientific research to study the function and role of CD93 in monkeys, which is a cell surface receptor involved in processes like angiogenesis and immune regulation. Researchers may use this cell line for experiments related to cell adhesion, signal transduction, or immune response modulation. It can serve as a valuable tool for investigating Monkey CD93-specific functions and interactions.

Are there any unique characteristics or properties associated with the Monkey CD93 Stable Cell Line - HEK293T compared to other CD93-expressing cell lines?

A: The Monkey CD93 Stable Cell Line - HEK293T may have specific advantages compared to other cell lines expressing CD93. These advantages could include a stable and consistent expression of Monkey CD93, a well-characterized background (HEK293T), and the potential for use in various assays and experiments. Researchers should consider these characteristics when choosing a cell line for their specific research needs.

What are the recommended culture conditions and media for maintaining the Monkey CD93 Stable Cell Line - HEK293T?

A: To maintain the Monkey CD93 Stable Cell Line - HEK293T, researchers should typically culture the cells in growth medium suitable for HEK293T cells, supplemented with the appropriate selection agent (e.g., neomycin). The specific culture conditions, such as temperature, CO2 concentration, and passage protocols, may depend on the established protocol used to generate and maintain this stable cell line. It's crucial to follow the instructions provided by the cell line provider or consult relevant literature for precise culture conditions.

What precautions should researchers take when working with the Monkey CD93 Stable Cell Line - HEK293T to ensure reliable and reproducible results?

A: When working with the Monkey CD93 Stable Cell Line - HEK293T, researchers should pay attention to several key factors: a. Regularly check and maintain the stability of Monkey CD93 expression. b. Ensure that the selection agent (e.g., neomycin) is present in the culture medium to prevent the loss of Monkey CD93-expressing cells. c. Perform proper quality control to confirm the identity and characteristics of the cell line. d. Avoid over-passage of the cells to prevent phenotypic changes. e. Document culture conditions and experimental procedures meticulously for reproducibility. f. Consider performing functional assays to validate Monkey CD93 functionality in the stable cell line.

Are there any known limitations or challenges associated with using the Monkey CD93 Stable Cell Line - HEK293T in research, such as potential cross-reactivity with human CD93 antibodies or issues related to species-specific differences?

A: Researchers should be aware of potential limitations when using the Monkey CD93 Stable Cell Line - HEK293T in research. These may include the possibility of cross-reactivity with antibodies designed for human CD93, as there could be differences in the antigenic regions between the monkey and human CD93 proteins. Additionally, researchers should consider that findings from experiments using this cell line may not always directly translate to humans due to species-specific differences. Careful validation and consideration of these factors are essential when interpreting results and drawing conclusions in a research context.

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