Lipopolysaccharides from S. minnesota R7

Name: Lipopolysaccharides from S. minnesota R7
SKU: VAR-018

For research use only. Not intended for any clinical use.

Cat.No.

VAR-018

Description

Lipopolysaccharide (LPS) is a natural adjuvant synthesized by gram-negative bacteria. LPS and its lipid A moiety can stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Product Type

Bacteria-derived Adjuvant

Purity

≥99%

Stability

Stable for at least 2 years after receipt when stored at 4°C.

Storage

Store at 4°C;

Publications

Q & A

Customer Reviews

Customer Q&As

How long can Lipopolysaccharides from S. minnesota R7 be stored?

A: A 1mg/ml solution in buffer or culture media is stable at 2-8°C for about one month. Frozen aliquots can be stored for up to two years. Avoid repeated freeze-thaw cycles.

What are the precautions for Lipopolysaccharides from S. minnesota R7?

A: If using glass containers, Lipopolysaccharides from S. minnesota R7 should be vortexed for at least 30 minutes to re-dissolve the adsorbed product. It is not recommended to undergo repeated freeze/thaw cycles.

What is the procedure for preparing Lipopolysaccharides from S. minnesota R7 upon receipt?

A: Lipopolysaccharides from S. minnesota R7 can be dissolved in water (2 mg/ml) or cell culture media (1 mg/ml), resulting in a cloudy pale yellow solution.

How should Lipopolysaccharides from S. minnesota R7 be used in cell culture?

A: For cell culture applications, reconstitute LPS by adding 1 ml of sterile balanced salt solution or culture media to 1 mg vial and gently swirling until the powder is dissolved. solution or culture media to the desired working concentration.

Can Lipopolysaccharides from S. minnesota R7 be diluted?

A: Dilution can be done as needed by further diluting the solution with sterile balanced salt

How is Lipopolysaccharides from S. minnesota R7 prepared?

A: Lipopolysaccharides from S. minnesota R7 are prepared from the Minnesota strain R7 (Rd mutant) of Salmonella.

Is Lipopolysaccharides from S. minnesota R7 only for laboratory use?

A: They are intended for laboratory use only and not for medical, household, or other purposes.

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