Lipopolysaccharides from E. coli EH100

Name: Lipopolysaccharides from E. coli EH100
SKU: VAR-007
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : VAR-007

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Product Information

Cat. No.	VAR-007
Description	Lipopolysaccharide (LPS) is a natural adjuvant synthesized by gram-negative bacteria. LPS and its lipid A moiety can stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Product Type	Bacteria-derived Adjuvant
Stability	Stable for at least 2 years after receipt when stored at 4°C.
Purity	≥99%
Storage	Store at 4°C;

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Customer Q&As

How was the reagent obtained?

A: This product is extracted from Escherichia coli EH100 (Ra mutant strain) using phenol:chloroform:petroleum ether.

What is the highest concentration that can be prepared?

A: It is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml), producing a cloudy pale yellow solution. It can be dissolved in saline and forms a higher concentration, but still cloudy solution (20 mg/ml) when vortexed and heated to 70-80°C.

Can organic solvents be used for dissolution?

A: It is not recommended to use organic solvents as they cannot form clearer solutions. Methanol, for example, can produce a cloudy suspension with floating particles, while water can produce a uniformly cloudy solution.

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Customer Reviews

Immune function

The product was successfully used to identify immune-related proteins.

United States

2020-01-12

Easy dissolution

Generally, dissolution can be achieved using water or culture medium.

United States

2021-04-11

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Lipopolysaccharides from E. coli EH100

Product Information

Q & A

Customer Reviews

How was the reagent obtained?

What is the highest concentration that can be prepared?

Can organic solvents be used for dissolution?

Immune function

Easy dissolution

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