Lipopolysaccharides from E. coli J5

Name: Lipopolysaccharides from E. coli J5
SKU: VAR-009

For research use only. Not intended for any clinical use.

Cat.No.

VAR-009

Description

Lipopolysaccharide (LPS) is a natural adjuvant synthesized by gram-negative bacteria. LPS and its lipid A moiety can stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Product Type

Bacteria-derived Adjuvant

Purity

≥99%

Stability

Stable for at least 2 years after receipt when stored at 4°C.

Storage

Store at 4°C;

Publications

Q & A

Customer Reviews

Customer Q&As

What is the source of Lipopolysaccharides from E. coli J5, including its extraction and preparation processes?

A: Lipopolysaccharides from E. coli J5 are typically extracted and prepared from the E. coli J5 strain. This involves culturing E. coli J5 and then extracting and purifying its lipopolysaccharides through cell disruption and separation techniques.

In which research areas, such as inflammation research or immunomodulation, is Lipopolysaccharides from E. coli J5 applied in cell lines?

A: Lipopolysaccharides from E. coli J5 are commonly applied in inflammation research and immunomodulation research. They are potent inflammatory stimulants used to simulate inflammatory responses in the immune system.

When using Lipopolysaccharides from E. coli J5, how is its concentration and appropriate dosage determined to ensure the accuracy of experimental results?

A: Determining the concentration and appropriate dosage of Lipopolysaccharides from E. coli J5 typically requires initial experiments and optimization. This includes using specific measurement methods to accurately measure its concentration and conducting experiments at different doses to determine the optimal dosage.

Is there variation in the response of Lipopolysaccharides from E. coli J5 in different types of cell lines, and how might this variation impact research?

A: Lipopolysaccharides from E. coli J5 may elicit varying responses in different types of cell lines. This variation can impact the interpretation and generalizability of research. Therefore, experiments in different cell lines are necessary to understand their effects.

How is the endotoxic effect of Lipopolysaccharides from E. coli J5 handled when using it to avoid adverse effects on cells?

A: To mitigate the endotoxic effect of Lipopolysaccharides from E. coli J5, methods such as endotoxin removal or neutralization are typically employed. This can include heat treatment, affinity chromatography, or the use of endotoxin-neutralizing agents to reduce adverse effects on cells.

What are the potential clinical applications of Lipopolysaccharides from E. coli J5, and are there known safety issues in its clinical use?

A: Lipopolysaccharides from E. coli J5 may have potential clinical applications, such as in immunotherapy. However, more clinical research is needed to assess its safety and potential adverse reactions. Known endotoxic effects may lead to serious adverse effects, necessitating measures to mitigate risks in its use.

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