Human NECTIN2 Stable Cell Line - HEK293T

Name: Human NECTIN2 Stable Cell Line - HEK293T
SKU: CSC-RO0894
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0894

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0894
Description	This cell line is engineered to stably overexpress human NECTIN2 (also named as PVRL2).
Target Gene	NECTIN2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What is the specific expression profile of the Human NECTIN2 Stable Cell Line - HEK293T?

A: The Human NECTIN2 Stable Cell Line - HEK293T is created by stably transfecting the NECTIN2 gene into HEK293T cells. In this cell line, the expression of NECTIN2 is artificially regulated, usually by using specific promoters. Given the high transfection efficiency and good growth characteristics of HEK293T cells, they are ideal for studying the function and regulatory mechanisms of NECTIN2 protein.

How is the Human NECTIN2 Stable Cell Line - HEK293T used in studies of cell adhesion and migration?

A: NECTIN2 is a cell adhesion molecule involved in interactions between various cells. In Human NECTIN2 Stable Cell Line - HEK293T, researchers can observe the effects of overexpression or knockdown of NECTIN2 on cell adhesion and migration, crucial for understanding biological processes such as cancer metastasis and cell communication.

What is the potential role of Human NECTIN2 Stable Cell Line - HEK293T in tumor immunology research?

A: NECTIN2 expression changes in various tumors may affect the ability of immune cells to recognize and kill tumor cells. With the Human NECTIN2 Stable Cell Line - HEK293T, researchers can explore the role of NECTIN2 in regulating immune escape, the tumor microenvironment, and immunotherapy strategies.

What factors should be considered when using Human NECTIN2 Stable Cell Line - HEK293T for drug screening?

A: When using the Human NECTIN2 Stable Cell Line - HEK293T for drug screening, the expression level of NECTIN2, cell growth characteristics, and the mechanism of action of the drug should be considered. Additionally, the specificity and toxicity of the drug towards NECTIN2 and other potential targets need to be evaluated.

What are the limitations of the Human NECTIN2 Stable Cell Line - HEK293T in simulating human diseases?

A: Although the Human NECTIN2 Stable Cell Line - HEK293T provides a platform for studying the function of NECTIN2, they are in vitro cultured human cells, which may not fully mimic the in vivo environment. Additionally, these cells are genetically modified, and their phenotype may differ from normal cells.

How is the Human NECTIN2 Stable Cell Line - HEK293T applied in the study of cell aging and death?

A: By observing the impact of NECTIN2 on aging and death of HEK293T cells under different physiological or stress conditions, the potential role of NECTIN2 in regulating the cell life cycle can be revealed. This is important for understanding the development of certain diseases.

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