Human LILRB3 Stable Cell Line - HEK293T

Name: Human LILRB3 Stable Cell Line - HEK293T
SKU: CSC-RO0934
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0934

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0934
Description	This cell line is engineered to stably overexpress human LILRB3.
Target Gene	LILRB3
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	LILRB3 leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3 [ Homo sapiens ]
Gene Symbol	LILRB3
Synonyms	HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; LILRA6
Gene Description	leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3
Gene ID	11025
Uni Prot ID	O75022
m RNA Refseq	NM_006864.2
Protein Refseq	NP_006855.2
Chromosome Location	19q13.4
Function	protein binding; receptor activity; transmembrane signaling receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem;
MIM	604820

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