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Cat.No. : CSC-RO0935 Host Cell: CHO-K1
| Cat. No. | CSC-RO0935 |
| Description | This cell line is engineered to stably overexpress human LILRB3. |
| Gene | LILRB3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: It was developed by transfecting the human CLEC9A gene into Chinese hamster ovary cells (CHO-K1) using genetic engineering techniques to achieve stable expression of the CLEC9A gene.
A: It is primarily used to study the role of CLEC9A in antigen capture, cross-presentation, and immune responses, especially in the research of tumor immunology and infectious diseases.
A: It should be cultured under aseptic conditions, maintaining appropriate temperature and pH levels, and regularly monitoring cell status and CLEC9A expression levels.
A: It can be used to test and develop immunotherapeutic drugs targeting CLEC9A, such as monoclonal antibodies for tumor immune responses.
A: Given the significance of CLEC9A in immune cell activation and tumor cell recognition, this cell line is crucial for studying its molecular mechanisms and signaling pathways.
A: It can be used to establish in vitro models of tumor immune responses and infectious diseases to study CLEC9A-mediated cellular immune responses.
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