Cat.No. : CSC-RO0664 Host Cell: HEK293T
| Cat. No. | CSC-RO0664 |
| Description | This cell line is engineered to overexpress human leukocyte immunoglobulin like receptor B2 (LILRB2). |
| Gene | LILRB2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The LILRB2 gene encodes for the leukocyte immunoglobulin-like receptor subfamily B member 2, also known as CD85j. This is a cell surface receptor expressed on myeloid cells, including monocytes and macrophages. LILRB2 plays a role in regulating immune responses by inhibiting the activation of T cells and natural killer cells.
A: The LILRB2 gene contributes to immune system regulation by binding to MHC class I molecules on the surface of antigen-presenting cells. This interaction inhibits the activation of T cells, thus preventing inappropriate immune responses and maintaining tolerance to self-antigens.
A: Mutations in the LILRB2 gene have been associated with various autoimmune diseases, where impaired function of the LILRB2 protein leads to an overactive immune response against the body's own tissues. Additionally, certain leukemias and lymphomas have been found to overexpress LILRB2, potentially influencing their pathogenesis.
A: The expression of the LILRB2 gene is regulated by various factors, including cytokines and transcription factors. For example, IL-6 and GM-CSF can upregulate LILRB2 expression in myeloid cells. The expression of LILRB2 is also influenced by epigenetic mechanisms, such as DNA methylation and histone modifications, which control its availability for transcription.
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The Human LILRB2 Stable Cell Line - HEK293T benefits from regular updates, ensuring that researchers have access to the latest advancements and improvements in cell line technology, enhancing the relevance and accuracy of their experiments.
Utilizing state-of-the-art editing technology, this cell line offers precise and efficient genetic modifications, allowing researchers to study LILRB2 with high accuracy and reliability, and opening up new possibilities for targeted research.
The Human LILRB2 Stable Cell Line - HEK293T employs a variety of editing strategies, providing researchers with flexibility and versatility in their experimental approaches, enabling them to explore different aspects of LILRB2 function and regulation.
With an extremely low risk of off-target effects, the Human LILRB2 Stable Cell Line - HEK293T ensures that researchers can confidently conduct their experiments without the concern of unintended genetic alterations, leading to more reliable and meaningful results.
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