Cat.No. : CSC-RO0663 Host Cell: 3A9
| Cat. No. | CSC-RO0663 |
| Description | This cell line is engineered to overexpress human leukocyte immunoglobulin like receptor B2 (LILRB2). |
| Gene | LILRB2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | 3A9 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human LILRB2 Stable Cell Line - 3A9 is primarily used in oncology, central nervous system research, immunological studies, and infectious disease research. This cell line is particularly suitable for drug screening and biological assays.
A: When culturing the Human LILRB2 Stable Cell Line - 3A9, it is necessary to maintain the conditions at 37°C and 5% CO2. The culture medium used is RPMI 1640 supplemented with 10% fetal bovine serum and 1μg/mL puromycin. This cell line grows in suspension and requires regular testing for mold, bacteria, and mycoplasma.
A: Human LILRB2 Stable Cell Line - 3A9 is a stable human cell line engineered to express the LILRB2 (Leukocyte Immunoglobulin-Like Receptor B2) gene, which is an immunoglobulin-like receptor typically expressed on the surface of immune cells and involved in immune regulation.
A: The cell culture conditions for Human LILRB2 Stable Cell Line - 3A9 typically involve the use of culture medium, temperature, CO2 concentration, and cell density. Cells are usually cultured at 37°C with 5% CO2 concentration using appropriate culture medium to support their growth and stability. Culture conditions may vary depending on the research purpose, so it is advisable to refer to relevant literature or recommendations from the cell line provider.
A: The stability and reliability of Human LILRB2 Stable Cell Line - 3A9 are typically influenced by multiple factors, including the maintenance and validation of the cell line. Maintaining the stability of the cell line involves regular passaging and checks to ensure stable expression of the LILRB2 gene. Additionally, validation methods such as Western blotting and flow cytometry can be used to confirm the presence and functionality of LILRB2 protein. The stability and reliability of the cell line are also influenced by culture conditions, so it is essential to follow standard culture protocols.
A: Human LILRB2 Stable Cell Line - 3A9 is typically prepared through genetic engineering techniques.
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