Human LILRB1 Stable Cell Line - HEK293T

Despite long-lived serotype-specific immunity following initial infection, the anticipated global prevalence of dengue now exceeds World Health Organization forecasts by more than thrice, with 390 million infections per year. The researchers show that antibody-opsonized DENV evades early antiviral responses by binding to the leukocyte Ig-like receptor-B1 (LILRB1) on monocytes, macrophages, and dendritic cells. This connection reduces FcγR signaling, lowering ISG production, which is essential for antiviral defense. LILRB1's role in recruiting Src homology phosphatase-1 to dephosphorylate Syk reveals a mechanism that allows for increased DENV replication upon secondary infection with a heterologous DENV serotype.

Figure 1. The researchers characterized LILRB1's role in ADE. They examined binding to DENV and DENV E protein, assessed plaque titers with soluble LILRB1 ectodomain or knockdown, and evaluated infections in monocytes and PBMCs, confirming its essential role in enhancing DENV infection. The extracellular domain of LILRB1 was cloned into pCMV-XL5, transfected into HEK293T cells for protein expression, purified, and incubated with DENV-2 or h3H5-opsonized DENV-2 for 1 hour at 37°C before addition to THP-1.2S cells. (Chan KR, et al., 2024)