Human KLRD1/KLRC1 Stable Cell Line - HEK293T

Name: Human KLRD1/KLRC1 Stable Cell Line - HEK293T
SKU: CSC-RO0896
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0896

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0896
Description	This cell line is engineered to stably overexpress human KLRD1/KLRC1 (also named as CD94-NKG2A).
Target Gene	KLRD1/KLRC1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What is the application of Human KLRD1/KLRC1 Stable Cell Line - HEK293T in immune detection?

A: The Human KLRD1/KLRC1 Stable Cell Line - HEK293T is significant in immune detection due to its stable expression of KLRD1 and KLRC1, which are important natural killer cell receptors. These cells can be used to study the role of these receptors in immune responses, particularly in the activation and inhibition of natural killer cells.

What are the challenges in gene editing of Human KLRD1/KLRC1 Stable Cell Line - HEK293T?

A: The main challenges in gene editing of Human KLRD1/KLRC1 Stable Cell Line - HEK293T include ensuring the stable expression of the KLRD1 and KLRC1 genes, avoiding non-specific gene interference, and maintaining the growth characteristics and biological activity of the HEK293T cells.

What role does Human KLRD1/KLRC1 Stable Cell Line - HEK293T play in drug screening?

A: Human KLRD1/KLRC1 Stable Cell Line - HEK293T can be used in drug screening, particularly for drugs affecting the activity of natural killer cells. By monitoring changes in signaling mediated by KLRD1 and KLRC1, potential immunomodulators can be identified.

Are there any special culture condition requirements for culturing Human KLRD1/KLRC1 Stable Cell Line - HEK293T?

A: Culturing Human KLRD1/KLRC1 Stable Cell Line - HEK293T requires maintaining specific environmental conditions, such as suitable temperature, pH, and adequate nutrients. Additionally, special growth factors or compounds may need to be added to maintain stable expression of KLRD1 and KLRC1.

What potential applications does Human KLRD1/KLRC1 Stable Cell Line - HEK293T have in disease research?

A: This cell line has potential applications in researching diseases related to natural killer cell functional disorders, such as certain cancers and autoimmune diseases. Analyzing the function of KLRD1 and KLRC1 can lead to a better understanding of the immune mechanisms of these diseases.

What are the characteristics of Human KLRD1/KLRC1 Stable Cell Line - HEK293T in cell signaling research?

A: The characteristic of Human KLRD1/KLRC1 Stable Cell Line - HEK293T in cell signaling research is the ability to directly observe and analyze the activation of KLRD1 and KLRC1 receptors and the changes in downstream signaling. This is crucial for understanding the role of natural killer cells in immune surveillance.

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