Cat.No. : CSC-RO0892 Host Cell: HEK293T
| Cat. No. | CSC-RO0892 |
| Description | This cell line is engineered to stably overexpress human KLRC1 (also named as NKG2A). |
| Gene | KLRC1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line can be used to investigate the mechanisms by which natural killer (NK) cells recognize and regulate target cells via the KLRC1 receptor (also known as NKG2A). It is particularly useful in exploring tumor immune escape and NK cell-mediated cytotoxic responses.
A: The Human KLRC1 Stable Cell Line - HEK293T is typically utilized for research related to the study of Natural Killer Cell Receptor Group 1 (KLRC1) and its associated cellular functions. Researchers may investigate KLRC1's role in immune responses and other biological processes using this cell line.
A: The Human KLRC1 Stable Cell Line - HEK293T offers the advantage of stable and controlled expression of KLRC1, which allows researchers to study its function in a consistent and reproducible manner. Additionally, HEK293T cells are commonly used for protein expression and have well-established protocols, making them a convenient choice for experiments involving KLRC1.
A: Yes, researchers often use specific antibodies against KLRC1 in immunoblotting, immunoprecipitation, and flow cytometry experiments to validate the expression and functionality of KLRC1 in the cell line.
A: Authentication of the cell line can be done through techniques like short tandem repeat (STR) profiling, and regular testing for mycoplasma contamination is essential to ensure purity. Proper storage and handling procedures should also be followed to maintain cell line authenticity.
A: KLRC1, also known as NKG2A, is a receptor expressed on the surface of natural killer (NK) cells and some T cells. It plays a crucial role in regulating immune responses by interacting with HLA-E molecules. Dysregulation of KLRC1 can impact immune surveillance and has implications in diseases such as cancer and viral infections.
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