Human KLRC1 Stable Cell Line - HEK293T

Name: Human KLRC1 Stable Cell Line - HEK293T
SKU: CSC-RO0892
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0892

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0892
Description	This cell line is engineered to stably overexpress human KLRC1 (also named as NKG2A).
Target Gene	KLRC1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KLRC1 killer cell lectin-like receptor subfamily C, member 1 [ Homo sapiens ]
Gene Symbol	KLRC1
Synonyms	KLRC1; killer cell lectin-like receptor subfamily C, member 1; NKG2; NKG2-A/NKG2-B type II integral membrane protein; CD159a; NKG2 1/B activating NK receptor; NKG2 A; NKG2 B; NK cell receptor A; C-lectin type II protein; natural killer cell lectin; natural killer group protein 2; NKG2-1/B activating NK receptor; NKG2-A/B-activating NK receptor; CD159 antigen-like family member A; NKG2-A/B type II integral membrane protein; NKG2A; CD159A; MGC13374; MGC59791;
Gene ID	3821
Uni Prot ID	P26715
m RNA Refseq	BC053840
Chromosome Location	12p13
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Graft-versus-host disease, organism-specific biosystem; Graft-versus-host disease, conserved biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem;
MIM	161555

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the specific application of the Human KLRC1 Stable Cell Line - HEK293T in studying NK cell activity?

A: This cell line can be used to investigate the mechanisms by which natural killer (NK) cells recognize and regulate target cells via the KLRC1 receptor (also known as NKG2A). It is particularly useful in exploring tumor immune escape and NK cell-mediated cytotoxic responses.

What is the specific purpose or research application for which the Human KLRC1 Stable Cell Line - HEK293T is commonly used?

A: The Human KLRC1 Stable Cell Line - HEK293T is typically utilized for research related to the study of Natural Killer Cell Receptor Group 1 (KLRC1) and its associated cellular functions. Researchers may investigate KLRC1's role in immune responses and other biological processes using this cell line.

What are the key advantages of using the Human KLRC1 Stable Cell Line - HEK293T in research compared to other available cell lines for studying KLRC1?

A: The Human KLRC1 Stable Cell Line - HEK293T offers the advantage of stable and controlled expression of KLRC1, which allows researchers to study its function in a consistent and reproducible manner. Additionally, HEK293T cells are commonly used for protein expression and have well-established protocols, making them a convenient choice for experiments involving KLRC1.

Are there any specific antibodies or markers commonly used to validate the expression of KLRC1 in the Human KLRC1 Stable Cell Line - HEK293T?

A: Yes, researchers often use specific antibodies against KLRC1 in immunoblotting, immunoprecipitation, and flow cytometry experiments to validate the expression and functionality of KLRC1 in the cell line.

How can researchers ensure the authenticity and purity of the Human KLRC1 Stable Cell Line - HEK293T to maintain the reliability of their experiments?

A: Authentication of the cell line can be done through techniques like short tandem repeat (STR) profiling, and regular testing for mycoplasma contamination is essential to ensure purity. Proper storage and handling procedures should also be followed to maintain cell line authenticity.

Can you explain the significance of KLRC1 in the context of immunology and its potential implications in disease research?

A: KLRC1, also known as NKG2A, is a receptor expressed on the surface of natural killer (NK) cells and some T cells. It plays a crucial role in regulating immune responses by interacting with HLA-E molecules. Dysregulation of KLRC1 can impact immune surveillance and has implications in diseases such as cancer and viral infections.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *