Human ENTPD1 Stable Cell Line - CT26

Name: Human ENTPD1 Stable Cell Line - CT26
SKU: CSC-RO0887
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0887

Host Cell : CT26 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0887
Description	This cell line is engineered to stably overexpress human ENTPD1 (also named as CD39).
Target Gene	ENTPD1
Gene Species	Homo sapiens (Human)
Host Cell	CT26
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	ENTPD1 ectonucleoside triphosphate diphosphohydrolase 1 [ Homo sapiens ]
Gene Symbol	ENTPD1
Synonyms	ENTPD1; ectonucleoside triphosphate diphosphohydrolase 1; CD39; ATPDase; NTPDase 1; CD39 antigen; ecto-apyrase; ecto-ATPase 1; ecto-ATPDase 1; ecto-ATP diphosphohydrolase 1; lymphoid cell activation antigen; NTPDase-1; FLJ40921; FLJ40959; DKFZp686D194; DKFZp686I093;
Gene ID	953
Uni Prot ID	P49961
m RNA Refseq	BC047664
Chromosome Location	10q23.1-q24.1
Function	ATP binding; hydrolase activity; nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; nucleotide binding;
Pathway	Purine metabolism, organism-specific biosystem; Purine metabolism, conserved biosystem; Pyrimidine metabolism, organism-specific biosystem; Pyrimidine metabolism, conserved biosystem;
MIM	601752

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Q & A

Customer Reviews

Customer Q&As

How was the Human ENTPD1 Stable Cell Line - CT26 developed?

A: This cell line was developed by stably transfecting the human ENTPD1 gene into the CT26 cell line, which is a mouse colon carcinoma cell line.

What are the main research applications of the Human ENTPD1 Stable Cell Line - CT26?

A: It is primarily used for studying the role of ENTPD1 in tumor biology, especially in research on the tumor immune environment and immune evasion mechanisms.

What factors need to be considered when culturing the Human ENTPD1 Stable Cell Line - CT26?

A: Attention should be given to maintaining appropriate culture conditions, such as temperature, CO2 concentration, culture medium, and minimizing the risk of contamination.

What are the specific applications of the Human ENTPD1 Stable Cell Line - CT26 in drug screening and evaluation?

A: It can be used for screening and evaluating drugs targeting ENTPD1, particularly in the fields of immunotherapy and cancer treatment.

What is the uniqueness of the Human ENTPD1 Stable Cell Line - CT26 in immune regulation research?

A: Due to the role of ENTPD1 in regulating immune responses and promoting tumor growth, this cell line is crucial for studying immune regulatory mechanisms.

What are the applications of the Human ENTPD1 Stable Cell Line - CT26 in cell signaling and tumor microenvironment research?

A: Useful for studying the role of ENTPD1 in tumor cell signaling and its impact on the tumor microenvironment.

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