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Cat.No. : CSC-RO0887 Host Cell: CT26
| Cat. No. | CSC-RO0887 |
| Description | This cell line is engineered to stably overexpress human ENTPD1 (also named as CD39). |
| Gene | ENTPD1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CT26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line was developed by stably transfecting the human ENTPD1 gene into the CT26 cell line, which is a mouse colon carcinoma cell line.
A: It is primarily used for studying the role of ENTPD1 in tumor biology, especially in research on the tumor immune environment and immune evasion mechanisms.
A: Attention should be given to maintaining appropriate culture conditions, such as temperature, CO2 concentration, culture medium, and minimizing the risk of contamination.
A: It can be used for screening and evaluating drugs targeting ENTPD1, particularly in the fields of immunotherapy and cancer treatment.
A: Due to the role of ENTPD1 in regulating immune responses and promoting tumor growth, this cell line is crucial for studying immune regulatory mechanisms.
A: Useful for studying the role of ENTPD1 in tumor cell signaling and its impact on the tumor microenvironment.
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