Human CLEC12A Stable Cell Line - CHO-K1

Name: Human CLEC12A Stable Cell Line - CHO-K1
SKU: CSC-RO0507
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0507

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0507
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Human CLEC12A.
Target Gene	CLEC12A
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CLEC12A C-type lectin domain family 12, member A [ Homo sapiens ]
Gene Symbol	CLEC12A
Synonyms	CLEC12A; C-type lectin domain family 12, member A; C-type lectin domain family 12 member A; CLL 1; MICL; C-type lectin superfamily; C-type lectin protein CLL-1; C-type lectin-like molecule-1; dendritic cell-associated lectin 2; myeloid inhibitory C-type lectin-like receptor; CLL1; CLL-1; DCAL-2; MGC70602;
Gene ID	160364
Uni Prot ID	Q5QGZ9
m RNA Refseq	BC063424
Chromosome Location	12p13.31
Function	binding; receptor activity; sugar binding;
MIM	612088

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the potential application of Human CLEC12A Stable Cell Line - CHO-K1 in studying tumor immune evasion?

A: Human CLEC12A Stable Cell Line - CHO-K1, with its stable expression of human C-type lectin-like receptor 12A (CLEC12A), holds significant potential in tumor immune evasion research. CLEC12A is expressed on the surface of various tumor cells and may play a role in inhibiting the immune system's recognition and elimination of tumor cells. This cell line can be used to study the mechanisms of CLEC12A in tumor immune evasion and its potential as a therapeutic target.

How can CLEC12A-mediated signal transduction be studied using Human CLEC12A Stable Cell Line - CHO-K1?

A: By using Human CLEC12A Stable Cell Line - CHO-K1, the interactions of CLEC12A with other proteins can be studied through immunoprecipitation and mass spectrometry, revealing the CLEC12A-mediated signaling pathways. Additionally, gene knockout or knock-in techniques can be used to further investigate the key components and functions of CLEC12A signaling pathways.

How can the application of Human CLEC12A Stable Cell Line - CHO-K1 in drug screening be optimized?

A: To optimize the application of Human CLEC12A Stable Cell Line - CHO-K1 in drug screening, a high-throughput screening platform can be established, combined with multiple detection methods of cell viability, apoptosis, and signal pathway activity, to screen for agonists or antagonists targeting CLEC12A. Moreover, regularly verifying the expression stability and response sensitivity of the cell line is essential to ensure the reliability of screening results.

What is the potential role of Human CLEC12A Stable Cell Line - CHO-K1 in studying chronic inflammatory diseases?

A: Human CLEC12A Stable Cell Line - CHO-K1 can be used to study the role of CLEC12A in chronic inflammatory diseases. As CLEC12A may play a role in immune regulation, this cell line helps to investigate its regulatory mechanisms in chronic inflammatory diseases, such as autoimmune diseases and certain infectious diseases, providing targets for new treatment methods.

What key factors should be considered when conducting gene editing experiments with Human CLEC12A Stable Cell Line - CHO-K1?

A: When using Human CLEC12A Stable Cell Line - CHO-K1 for gene editing experiments, it is important to ensure the specificity and efficiency of gene editing tools, like CRISPR/Cas9. Detailed clone selection and gene expression analysis should be conducted to verify the accuracy of gene editing. Additionally, monitoring potential off-target editing and changes in cell phenotype is also crucial.

What are the prospects of Human CLEC12A Stable Cell Line - CHO-K1 in cell communication research?

A: Human CLEC12A Stable Cell Line - CHO-K1 shows promising applications in cell communication research. As a receptor, CLEC12A may be involved in regulating intercellular signal transduction, especially in interactions between immune cells and tumor or inflammatory cells. Studying these interactions can help understand the role of CLEC12A in cell communication, providing clues for developing new therapeutic strategies.

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