Cat.No. : CSC-RO0507 Host Cell: CHO-K1
| Cat. No. | CSC-RO0507 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Human CLEC12A. |
| Gene | CLEC12A |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human CLEC12A Stable Cell Line - CHO-K1, with its stable expression of human C-type lectin-like receptor 12A (CLEC12A), holds significant potential in tumor immune evasion research. CLEC12A is expressed on the surface of various tumor cells and may play a role in inhibiting the immune system's recognition and elimination of tumor cells. This cell line can be used to study the mechanisms of CLEC12A in tumor immune evasion and its potential as a therapeutic target.
A: By using Human CLEC12A Stable Cell Line - CHO-K1, the interactions of CLEC12A with other proteins can be studied through immunoprecipitation and mass spectrometry, revealing the CLEC12A-mediated signaling pathways. Additionally, gene knockout or knock-in techniques can be used to further investigate the key components and functions of CLEC12A signaling pathways.
A: To optimize the application of Human CLEC12A Stable Cell Line - CHO-K1 in drug screening, a high-throughput screening platform can be established, combined with multiple detection methods of cell viability, apoptosis, and signal pathway activity, to screen for agonists or antagonists targeting CLEC12A. Moreover, regularly verifying the expression stability and response sensitivity of the cell line is essential to ensure the reliability of screening results.
A: Human CLEC12A Stable Cell Line - CHO-K1 can be used to study the role of CLEC12A in chronic inflammatory diseases. As CLEC12A may play a role in immune regulation, this cell line helps to investigate its regulatory mechanisms in chronic inflammatory diseases, such as autoimmune diseases and certain infectious diseases, providing targets for new treatment methods.
A: When using Human CLEC12A Stable Cell Line - CHO-K1 for gene editing experiments, it is important to ensure the specificity and efficiency of gene editing tools, like CRISPR/Cas9. Detailed clone selection and gene expression analysis should be conducted to verify the accuracy of gene editing. Additionally, monitoring potential off-target editing and changes in cell phenotype is also crucial.
A: Human CLEC12A Stable Cell Line - CHO-K1 shows promising applications in cell communication research. As a receptor, CLEC12A may be involved in regulating intercellular signal transduction, especially in interactions between immune cells and tumor or inflammatory cells. Studying these interactions can help understand the role of CLEC12A in cell communication, providing clues for developing new therapeutic strategies.
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