Human CD93 Stable Cell Line - CHO-K1

Name: Human CD93 Stable Cell Line - CHO-K1
SKU: CSC-RO0858
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0858

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0858
Description	This cell line is engineered to stably overexpress human CD93.
Target Gene	CD93
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD93 CD93 molecule [ Homo sapiens ]
Gene Symbol	CD93
Synonyms	C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1
Gene Description	CD93 molecule
Gene ID	22918
Uni Prot ID	Q9NPY3
m RNA Refseq	NM_012072.3
Protein Refseq	NP_036204.2
Chromosome Location	20p11.21
Function	calcium ion binding; carbohydrate binding; complement component C1q binding; protein binding; receptor activity;
MIM	120577

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the Human CD93 Stable Cell Line - CHO-K1 constructed, and what are the key factors to consider during its construction?

A: The Human CD93 Stable Cell Line - CHO-K1 is constructed through genetic engineering techniques, where the human CD93 gene is introduced into CHO-K1 cells to enable stable expression of the CD93 protein. During the construction process, it is important to select an appropriate expression vector, optimize transfection conditions, and screen for cell clones that stably express the target protein. In addition, long-term culture and observation of cells are required to ensure their stability and expression level.

How to optimize culture conditions to improve the yield when using Human CD93 Stable Cell Line - CHO-K1 for bioproduct production?

A: To improve the yield of bioproduct production using Human CD93 Stable Cell Line - CHO-K1, the following aspects can be optimized: 1) Optimize the composition of the culture medium, such as adding appropriate growth factors, nutrients, and inducers; 2) Control the culture environment, such as temperature, pH, and dissolved oxygen; 3) Choose the appropriate culture mode, such as batch culture, continuous culture, or perfusion culture; 4) Regularly monitor cell density and bioproduct concentration to adjust the culture strategy in time.

What are the potential applications of Human CD93 Stable Cell Line - CHO-K1 in immunotherapy research?

A: Human CD93 Stable Cell Line - CHO-K1 has a wide range of potential applications in immunotherapy research. For example, it can be used to produce bioproducts with immunomodulatory functions, such as antibodies, cytokines, and fusion proteins; in addition, it can be used to study the role of CD93 in immune cell interactions, signal pathway regulation, and immune responses, providing new targets and strategies for immunotherapy.

How to evaluate the stability and expression level of Human CD93 Stable Cell Line - CHO-K1?

A: To evaluate the stability and expression level of Human CD93 Stable Cell Line - CHO-K1, the following methods can be used: 1) Detect the expression level of CD93 protein by Western blot or enzyme-linked immunosorbent assay (ELISA); 2) Analyze the expression of CD93 protein on the cell surface by flow cytometry (FACS); 3) Perform long-term culture of cells to observe changes in their growth characteristics and expression levels; 4) Compare different batches of cells to evaluate their stability and consistency.

What are the advantages of using Human CD93 Stable Cell Line - CHO-K1 for virus vector production?

A: There are several advantages to using Human CD93 Stable Cell Line - CHO-K1 for virus vector production: 1) CHO-K1 cells have a high transfection efficiency, which is beneficial for the efficient production of virus vectors; 2) This cell line has good tolerance to the production and release of virus vectors; 3) By optimizing culture conditions and processes, large-scale and high-density cell culture can be achieved, further increasing the yield of virus vectors.

How to avoid cell contamination when using Human CD93 Stable Cell Line - CHO-K1 for experiments?

A: To avoid cell contamination when using Human CD93 Stable Cell Line - CHO-K1 for experiments, the following measures can be taken: 1) Strictly follow aseptic operation procedures, such as using a sterile workbench, wearing sterile gloves and masks; 2) Regularly disinfect the laboratory environment and equipment; 3) Use sterile culture medium and reagents; 4) Regularly perform sterility tests on cells, such as microscopic examination and bacterial culture; 5) Avoid cross-contamination between different experiments by using dedicated laboratory equipment and instruments.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *