Cat.No. : CSC-RO0858 Host Cell: CHO-K1
| Cat. No. | CSC-RO0858 |
| Description | This cell line is engineered to stably overexpress human CD93. |
| Gene | CD93 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CD93 Stable Cell Line - CHO-K1 is constructed through genetic engineering techniques, where the human CD93 gene is introduced into CHO-K1 cells to enable stable expression of the CD93 protein. During the construction process, it is important to select an appropriate expression vector, optimize transfection conditions, and screen for cell clones that stably express the target protein. In addition, long-term culture and observation of cells are required to ensure their stability and expression level.
A: To improve the yield of bioproduct production using Human CD93 Stable Cell Line - CHO-K1, the following aspects can be optimized: 1) Optimize the composition of the culture medium, such as adding appropriate growth factors, nutrients, and inducers; 2) Control the culture environment, such as temperature, pH, and dissolved oxygen; 3) Choose the appropriate culture mode, such as batch culture, continuous culture, or perfusion culture; 4) Regularly monitor cell density and bioproduct concentration to adjust the culture strategy in time.
A: Human CD93 Stable Cell Line - CHO-K1 has a wide range of potential applications in immunotherapy research. For example, it can be used to produce bioproducts with immunomodulatory functions, such as antibodies, cytokines, and fusion proteins; in addition, it can be used to study the role of CD93 in immune cell interactions, signal pathway regulation, and immune responses, providing new targets and strategies for immunotherapy.
A: To evaluate the stability and expression level of Human CD93 Stable Cell Line - CHO-K1, the following methods can be used: 1) Detect the expression level of CD93 protein by Western blot or enzyme-linked immunosorbent assay (ELISA); 2) Analyze the expression of CD93 protein on the cell surface by flow cytometry (FACS); 3) Perform long-term culture of cells to observe changes in their growth characteristics and expression levels; 4) Compare different batches of cells to evaluate their stability and consistency.
A: There are several advantages to using Human CD93 Stable Cell Line - CHO-K1 for virus vector production: 1) CHO-K1 cells have a high transfection efficiency, which is beneficial for the efficient production of virus vectors; 2) This cell line has good tolerance to the production and release of virus vectors; 3) By optimizing culture conditions and processes, large-scale and high-density cell culture can be achieved, further increasing the yield of virus vectors.
A: To avoid cell contamination when using Human CD93 Stable Cell Line - CHO-K1 for experiments, the following measures can be taken: 1) Strictly follow aseptic operation procedures, such as using a sterile workbench, wearing sterile gloves and masks; 2) Regularly disinfect the laboratory environment and equipment; 3) Use sterile culture medium and reagents; 4) Regularly perform sterility tests on cells, such as microscopic examination and bacterial culture; 5) Avoid cross-contamination between different experiments by using dedicated laboratory equipment and instruments.
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