Human CD33 Stable Cell Line - CHO-K1

Name: Human CD33 Stable Cell Line - CHO-K1
SKU: CSC-RO0481
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0481

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0481
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Human CD33.
Target Gene	CD33
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD33 CD33 molecule [ Homo sapiens ]
Gene Symbol	CD33
Synonyms	p67; SIGLEC3; SIGLEC-3
Gene Description	CD33 molecule
Gene ID	945
Uni Prot ID	C9JEN7
m RNA Refseq	NM_001082618.1
Protein Refseq	NP_001076087.1
Chromosome Location	19q13.3
Function	carbohydrate binding; receptor activity;
Pathway	Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem;
MIM	159590

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Q & A

Customer Reviews

Customer Q&As

What is the role of the Human CD33 Stable Cell Line - CHO-K1 in studying the development of myeloid cells?

A: The Human CD33 Stable Cell Line - CHO-K1 cell line, by stably expressing CD33, a surface marker of myeloid cells, provides a model system for studying the development and differentiation of myeloid cells. This cell line can help scientists understand the function of CD33 during myeloid cell maturation and how it affects cell signaling and immune responses.

How does the Human CD33 Stable Cell Line - CHO-K1 contribute to exploring the molecular mechanisms of myelodysplastic syndrome (MDS)?

A: Myelodysplastic syndrome (MDS) is a disease affecting hematopoietic stem cells, leading to abnormal bone marrow function. The Human CD33 Stable Cell Line - CHO-K1 can simulate abnormal myeloid cell development, helping researchers identify molecular changes associated with MDS. This cell line can be used to screen drugs that may affect CD33 expression and function, providing potential targets for MDS treatment.

What is the application of the Human CD33 Stable Cell Line - CHO-K1 in developing targeted therapies for myeloid leukemia?

A: Myeloid leukemia is a cancer originating from myeloid cells. The Human CD33 Stable Cell Line - CHO-K1 can be used to study the role of CD33 in leukemia cells and how targeting CD33 can inhibit the growth and spread of leukemia cells. This cell line can serve as a model for drug screening and evaluation, aiding in the development of new leukemia treatments.

How does the Human CD33 Stable Cell Line - CHO-K1 assist in studying the role of CD33 in immune regulation?

A: CD33 plays a crucial role in immune regulation, particularly in the activation and function of myeloid cells. The Human CD33 Stable Cell Line - CHO-K1 can provide a platform to study its regulatory role in immune responses. This helps reveal how CD33 affects inflammatory responses and the activation state of immune cells.

What is the contribution of the Human CD33 Stable Cell Line - CHO-K1 in studying the pharmacodynamics of CD33 antibody drugs?

A: The Human CD33 Stable Cell Line - CHO-K1 can be used to evaluate the pharmacodynamic properties of antibody drugs targeting CD33. This cell line can simulate CD33 expression on human cells, helping researchers understand how these drugs bind to CD33 and assess their impact on cell function, which is crucial for drug development and clinical application.

How does the Human CD33 Stable Cell Line - CHO-K1 help in studying the role of CD33 in the tumor microenvironment?

A: Myeloid cells in the tumor microenvironment, including macrophages and dendritic cells, can interact with tumor cells through CD33. The Human CD33 Stable Cell Line - CHO-K1 can simulate these interactions, helping researchers understand the role of CD33 in tumor immune evasion and regulation of the tumor microenvironment. This is significant for developing new cancer immunotherapies.

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