Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : PTAK-029
| Cat. No. | PTAK-029 |
| Description | The HRV 3C Activity Assay Kit provides a convenient method for detecting HRV 3C Protease activity in mammalian tissue samples, biological fluids and from purified proteins. The assay kit is based on the ability of a 3C Protease (derived from a HRV rhinovirus-14) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity from as low as 50 ng protein present in samples. |
| Component |
•Active HRV3C protease •HRV3C protease substrate •HRV3C assay buffer •pAN standard |
| Applications | Useful for detecting the activity of HRV3C protease and screening/characterizing the potential inhibitors of HRV3C. |
| Size | 100 assays |
| Method | Colorimetric |
| Caution | Avoid freeze/thaw cycles |
A: HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
A: Proteolytic cleavage occurs between the Gln and Gly residues.
A: HRV3C protease is often used for protein purification and tag removal in recombinant protein expression systems.
A: HRV3C Protease is a 47 kDa protein.
A: HRV3C protease is engineered to have enhanced stability and specificity, making it a popular choice in laboratory applications.
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