EF1a-SV40/LTA(RFP, Puro) Lentiviral Particles

EF1a-SV40/LTA (RFP, Puro) Lentiviral Particles are high-titer, replication-deficient viral vectors designed for the robust and stable expression of the Simian Virus 40 Large T Antigen (LTA). Driven by the constitutive human Elongation Factor 1 alpha (EF1a) promoter, these particles ensure high-level transgene expression across a diverse range of mammalian cells, including primary cells and stem cells that are often resistant to CMV-driven expression. The vector architecture incorporates a dual-functional cassette containing Red Fluorescent Protein (RFP) for real-time monitoring of transduction efficiency and a Puromycin resistance marker to facilitate the rapid selection of stable cell populations. As a third-generation lentiviral system, these particles offer enhanced safety and high integration efficiency, making them a reliable choice for long-term genetic modification projects.

In terms of application, these lentiviral particles are predominantly utilized for cell immortalization and the establishment of stable cell lines. The SV40 Large T Antigen is a potent transforming protein that induces cell proliferation and bypasses replicative senescence by sequestering the p53 and Retinoblastoma (Rb) tumor suppressor proteins. This allows researchers to generate continuous cell lines from primary tissues, which is essential for studying complex cellular mechanisms, performing large-scale drug screening, and conducting functional genomic assays. Additionally, the combination of RFP and Puromycin selection makes these particles highly effective for multi-gene expression studies and high-throughput validation. Whether used for basic research in cell cycle regulation or applied biotechnology in protein production, these particles provide a streamlined workflow for engineering resilient and well-characterized cellular models.