EF1a-SV40/LTA(RFP, Bla) Lentiviral Particles

The EF1a-SV40/LTA (RFP, Bla) Lentiviral Particles represent a sophisticated, third-generation delivery system optimized for the stable and high-level expression of the SV40 Large T Antigen. Driven by the human Elongation Factor 1-alpha (EF1a) promoter, this vector ensures robust and persistent transgene activity across a broad spectrum of mammalian cell types, including primary cells and stem cells that are often resistant to traditional CMV-driven expression. The construct is engineered with a dual-reporter and selection configuration: Red Fluorescent Protein (RFP) enables non-invasive, longitudinal monitoring of transduction efficiency via fluorescence microscopy, while the Blasticidin (Bla) resistance marker allows for the rapid generation of uniform, stable cell populations through antibiotic selection. These replication-deficient particles are produced with high titers, ensuring efficient integration into the host genome while maintaining a high standard of laboratory biosafety.

These lentiviral particles are extensively employed in cell biology research, particularly for the immortalization of primary cell lines. By sequestering tumor suppressor proteins such as p53 and pRb, the SV40 Large T Antigen allows primary cells to bypass replicative senescence, providing researchers with a continuous and consistent cell source for long-term functional studies. Additionally, these particles are invaluable in bioproduction and pharmaceutical research; they facilitate the creation of high-yield producer cell lines by promoting the episomal replication of SV40-based plasmids. The ability to track transduced cells via RFP makes them ideal for co-culture experiments, lineage tracing, and high-throughput drug screening assays where visual verification of gene delivery is critical. From investigating viral oncogenesis to developing specialized cellular models for vaccine testing, these particles serve as a versatile tool for advancing both fundamental and applied biomedical sciences.