DPP4 Activity Assay Kit

Name: DPP4 Activity Assay Kit
SKU: PTAK-022

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-022

Description

The DPP4 Activity Assay Kit is designed to measure DPP4 activity using purified DPP4 for screening and profiling applications. The key to the Fluorogenic DPP4 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP4 reactions; no time-consuming washing steps are required. The DPP fluorometric substrate is incubated with a sample containing DPP4 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.

Applications

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Caution

Avoid freeze/thaw cycles

Component

•Active DPP4
•DPP4 fluorogenic substrate
•DPP4 assay buffer
•Fluorogenic AMC standard
•Microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is DPP4?

A: Dipeptidyl peptidase-4 (DPP4 or DPPIV), also known as adenosine deaminase complex protein 2 or CD26 (cluster of differentiation 26), is the protein encoded by the DPP4 gene in humans.

Where is DPP4 primarily expressed?

A: DPP4, or dipeptidyl peptidase 4, is an enzyme that is found on the surface of many cell types, including immune cells, epithelial cells, and endothelial cells.

What is the role of DPP4 in glucose metabolism?

A: DPP4 plays a role in the regulation of blood glucose levels by breaking down incretin hormones, such as glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP).

What substrates can DPP-4 cleave?

A: DPP-4 can cleave a broad range of substrates including growth factors, chemokines, neuropeptides, and vasoactive peptides

Can DPP4 be used as a target for treating diabetes?

A: Yes. By inhibiting DPP4, the activity of these incretins is prolonged, leading to increased insulin secretion, decreased glucagon release, and improved glycemic control. Therefore, DPP4 inhibitors are a type of medication used in the treatment of type 2 diabetes.

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