Dog CD47 Stable Cell Line - CHO-K1

Name: Dog CD47 Stable Cell Line - CHO-K1
SKU: CSC-RO0279
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0279

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0279
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress Dog CD47.
Target Gene	CD47
Gene Species	Canis lupus familiaris (Dog)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

When culturing Dog CD47 Stable Cell Line - CHO-K1, is there a need for special animal-derived components to maintain the functional activity of CD47 protein?

A: Typically, to maintain the functional activity of CD47 protein, a medium containing specific animal-derived components might be needed, or suitable culture conditions should be chosen based on the experimental purpose.

How to evaluate the similarities and differences in function between the canine CD47 protein in Dog CD47 Stable Cell Line - CHO-K1 and human or other species' CD47 protein?

A: Cross-reactivity experiments like immunoblotting and immune cell functional assays, as well as comparisons of CD47 protein sequences and structures, can be conducted to evaluate the functional similarities and differences between canine CD47 protein and human or other species' CD47 protein.

Is Dog CD47 Stable Cell Line - CHO-K1 suitable for studying canine disease models, such as certain immune-mediated diseases?

A: Yes, Dog CD47 Stable Cell Line - CHO-K1 may be suitable for studying canine disease models, especially when researching immune-mediated diseases related to CD47 protein function.

In Dog CD47 Stable Cell Line - CHO-K1, are there specific cellular signaling pathways activated by canine CD47 protein?

A: To identify specific cellular signaling pathways, signal pathway inhibitors, gene silencing or overexpression experiments can be performed to analyze the signaling transduction of canine CD47 protein in Dog CD47 Stable Cell Line - CHO-K1.

Can the canine CD47 protein in Dog CD47 Stable Cell Line - CHO-K1 cross-react with SIRPα ligands from other species, and what experimental methods can be used to validate this?

A: In vitro binding assays and immunoprecipitation experiments can be conducted to evaluate the cross-reactivity of canine CD47 protein with SIRPα ligands from other species.

In Dog CD47 Stable Cell Line - CHO-K1, how to evaluate the impact of potential drugs on the expression and function of canine CD47 protein, thereby providing a basis for the treatment of canine immune diseases?

A: Cellular functional assays post drug treatment, like cell adhesion and immune cell activation assays, along with the detection of CD47 protein expression levels through Western Blot and flow cytometry analyses, can be conducted to evaluate the impact of potential drugs on the expression and function of canine CD47 protein.

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