CMV-SV40/LTA(Neo) Lentiviral Particles

CMV-SV40/LTA(Neo) Lentiviral Particles are high-titer, replication-deficient viral vectors designed for the stable expression of the Simian Virus 40 Large T Antigen (LTA). This system utilizes the robust Cytomegalovirus (CMV) immediate-early promoter to drive high levels of constitutive expression in a wide array of mammalian cell types. The lentiviral delivery mechanism ensures that the SV40 LTA gene is integrated into the host cell genome, providing a permanent genetic modification that remains stable across multiple cell divisions. To assist in the development of uniform cell populations, the particles include a Neomycin resistance marker, which enables the efficient selection of successfully transduced cells through G418 treatment. Unlike standard transfection methods, these lentiviral particles can effectively infect difficult-to-transduce cells, including both dividing and non-dividing primary cultures, making them a versatile tool for long-term genetic studies.

The primary application of these particles is the immortalization of primary mammalian cells. By sequestering and inhibiting the functions of the p53 and retinoblastoma (Rb) tumor suppressor proteins, the SV40 Large T Antigen allows primary cells to bypass replicative senescence and proliferate indefinitely. This capability is crucial for researchers who need to establish stable, continuous cell lines from limited or rare tissue samples while preserving the essential phenotypic traits of the original cells. Beyond immortalization, these particles are frequently used in the production of recombinant proteins and viral vectors, where the presence of the T-antigen can enhance the replication of SV40 origin-containing plasmids. They also serve as a valuable model system for studying oncogenic transformation, cell cycle control, and the molecular pathways involved in DNA replication. The combination of high transduction efficiency and the convenience of Neomycin selection makes these particles an essential resource for creating reliable and reproducible cellular models for biomedical research.