CMV-SV40/LTA(GFP, Bla) Lentiviral Particles

CMV-SV40/LTA(GFP, Bla) lentiviral particles are cutting-edge tools in gene manipulation and cell engineering. These genetically engineered particles deliver the SV40 large T antigen (LTA) gene under the control of a potent CMV promoter, ensuring high levels of constitutive expression in transduced cells. A key advantage of this lentiviral system lies in its dual-function design: it integrates a GFP reporter gene for real-time visualization of transduction efficiency and cell localization, and also integrates a blastcin resistance gene (Bla) to facilitate screening for stably transduced cells via antibiotics. Furthermore, the lentiviral vector backbone offers other advantages, such as efficient integration into both dividing and non-dividing cells, broad tropism across various mammalian cell types, and stable long-term transgenic expression—all of which outperform transient transfection methods.

This multifunctional lentiviral system is particularly important in cell immortalization research. SV40 LTA can inactivate tumor suppressor pathways (such as p53 and Rb), thereby promoting the generation of proliferating cell lines from finite-lived primary tissues. These immortalized cells retain key physiological characteristics, enabling long-term experiments such as drug screening, toxicology assessment, and cellular pathway mechanism studies—experiments impossible in senescent primary cells. In addition to immortalization, these viral particles also support gene function analysis in difficult-to-transfect cells (such as neurons or stem cells), the construction of reporter gene cell lines for high-content screening, and the creation of disease models (where sustained LTA expression mimics oncogenic transformation).